血小板源性生长因子B在损伤周围神经再生中的作用

目的：观察血小板源性生长因子B（PDGF-B）在损伤大鼠坐骨神经中的表达，旨在阐明其在末梢神经再生中的作用。 方法：分别构建大鼠坐骨神经切割伤和碾压伤模型，应用免疫组织化学S-P法检测PDGF-B的表达。结果：两类损伤模型中，损伤近端神经和再生轴突PDGF-B的表达均增强。两类损伤模型均可见损伤神经远端的雪旺氏细胞PDGF-B表达量明显增加，在碾压伤模型中，随着轴突-雪旺氏细胞关系的恢复，PDGF-B表达水平下降，并最终恢复正常；在切割伤模型中，二者关系不能恢复，PDGF-B减至极低水平。 结论：坐骨神经损伤后PDGF-B的表达增强在末梢神经再生中发挥重要作用。

Role of platelet-derived growth factor-B in regeneration of injured peripheral nerve

ObjectiveTo observe the role of platelet derived growth factor B (PDGF B) in the regeneration of peripheral nerve by studying its expression on injured rat sciatic nerves. MethodsThe rat sciatic nerves cut and crush injured models were established, the expressions of PDGF B on both injured nerves were detected by immunohistochemistry technique. ResultsThe expressions of PDGF B were increased in proximal nerve segments and regenerating axons in both models. Soon after both types of injury, considerable amounts of PDGF B accumulated in Schwann cells in distal segments of both models. With restoration of the axon Schwann cells relationship in the crush model, levels of PDGF B tended to decrease, eventually returned to normal. In the cut model the relationship can not be restored, the PDGF B was decreased to a very low level. ConclusionThe increased expression of PDGF B after sciatic nerve injury may contribute to peripheral nerve regeneration.