不同产地野菊花中绿原酸、4, 5-二咖啡酰奎尼酸的含量测定

目的建立不同产地野菊花中绿原酸、4,5-二咖啡酰奎尼酸的含量测定方法。方法采用HPLC,色谱柱为DiamonsilC18(4.6 mm×250 mm,5μm);采用梯度洗脱;检测波长327 nm;柱温30℃;流速1.0 mL.min 1。结果绿原酸、4,5-二咖啡酰奎尼酸的线性范围分别为0.011～0.22μg,0.017 5～0.35μg。加样回收率分别为99.1%和98.8%;RSD分别为1.93%和1.54%(n=9)。结论本方法操作简便,分析速度快,结果准确,重复性好,可用于野菊花中绿原酸和4,5-二咖啡酰奎尼酸含量的测定。

Content Determination of Chlorogenic Acid and 4,5-O-Dicaffeoylquinic Acid in Chrysanthemi Indici Flos from Different Areas

OBJECTIVE To determine the content of chlorogenic acid and 4,5-O-dicaffeoylquinic acid in Chrysanthemi Indici Flos from different areas.METHODS HPLC was used.The chromatographic separation was performed on a Diamonsil C18（4.6 mm × 250 mm,5 μm） column with gradient elute.The detection wavelength was 327 nm and column temperature was 30 ℃.RESULTS Good linear relationship between the peak area and content of chlorogenic acid and 4,5-O-dicaffeoylquinic acid was achieved when the amount was within 0.011 0.22 μg,0.0175 0.35 μg;the recoveries were 99.1% and 98.8%;RSD were 1.93% and 1.54%（n=9）.CONCLUSION The method is accurate,reproducible and suitable to determine chlorogenic acid and 4,5-O-dicaffeoylquinic acid in Chrysanthemi Indici Flos.