一氧化氮吸入对缺氧性肺动脉高压患者一氧化氮合酶和内皮素-1的影响

目的: 探讨吸入一氧化氮(NO)对缺氧性肺动脉高压患者一氧化氮合酶(NOS)和内皮素-1(ET-1)的影响。方法: 13例肺动脉高压患者行NO吸入,分别于吸入NO前、吸入30 min时、停止吸入2 h和12 h采肺动脉血,测白细胞中的NOS及血浆中的ET-1。结果: 吸入NO前、吸入30 min时、停止吸入2 h和12 h,所测NOS值分别为(0.70±0.21)mol/min·mg^-1、(0.74±0.14)mol/min·mg^-1、(0.64±0.22)mol/min·mg^-1和(0.63±0.17)mol/min·mg^-1;所测ET-1为(78.89±46.59)pmol/L、(88.27±45.41)pmol/L、(80.76±42.66)pmol/L和(61.07±29.44)pmol/L;后三者同吸入前比,P均＞0.05。结论: 缺氧性肺动脉高压患者吸入NO对机体NOS和ET-1未见明显影响。

Effects of nitric oxide inhalation on nitric oxide synthase and endothelin-1 in patients with hypoxic pulmonary hypertension

AIM: To investigate the effects of nitric oxide (NO) inhalation on nitric oxide synthase (NOS) and endothelin-1 (ET-l) of patients with hypoxic pulmonary hypertension. METHODS: Examined 13 pulmonic blood samples to determine the concentration of NOS in leukocyte and ET-1 in plasma before NO inhalation, 30 minutes after inhalation, 2 and 12 hours after stopping of inhalation respectiviy. RESULTS: The values taken before inhalation was NOS (0.70 ± 0.21 )mol/min·mg^-1, ET-1 (78.89 ± 46.59) Pmol/L; 30 minutes after inhalation (0.74±0.14)mol/min·mg^-1, ET-1 (88.27 ± 45.41 )pmol/L; 2 hours after stopping of inhalation NOS (0.64 ± 0.22)mol/min·mg-1, ET-1 (80.76±42.66)pmol/L; and 12 hours after stopping of inhalation NOS (0. 63± 0. 17)mol/min.mg-1, ET-1(61.07±29.44)pmol/L. NO significant difference was found in the values of NOS and ET- 1 before and after inhalation, P＞ 0.05. CONCLUSION: The effects of NO inhalation on NOS and ET-l in patients with hypoxic pulmonary hypertension are not significant according to the above investigation.