黄芪对IEC-6肠上皮细胞缺氧复氧损伤保护效应的血清药理学研究

目的:探讨黄芪对IEC-6肠上皮细胞缺氧复氧损伤的保护效应.方法:建立IEC-6细胞缺氧复氧损伤模型;采用血清药理学方法,在细胞培养液中加入不同给药剂量制备的黄芪药物血清[一次和间隔1 h两次给药10 g/kg(DJ-1及SJ-1组),一次和间隔1 h两次给药20 g/kg(DJ-2及SJ-2组)]、参附药物血清(间隔1 h两次给药20 g/kg,SF组)及对照血清.应用噻唑蓝(MTT)法测定LDH漏出量和其细胞生长曲线.结果:与对照组比较,间隔1 h两次给药(10 g/kg,20 g/kg)1 h后制备的药物血清能明显减少IEC-6细胞LDH的漏出量,其中SJ-1组的P＜0.05,SJ-2组的P＜0.01;SJ-2组24 h细胞活力明显高于缺氧复氧细胞损伤组及参附血清组(P＜0.05),并随时间增加而逐渐增强.结论:黄芪对IEC-6肠上皮细胞缺氧复氧损伤具有显著保护效应.

A Serum Pharmacological Study on Protective Effect of Radix Astragali Liquid Treatment on Injury of Intestinal Epithelial Cell Induced by Hypoxia Reoxygenation

Objective:To study the protective effect of astraglus membranceous liquid on injury of intestinal epithelial cell induced by hypoxia reoxygenation.Methods:To use serum pharmacological method and to prepare different quantity of drug's serum.Cultured intestinal epithelial cell-6(IEC-6) cells were divided into seven groups(n=8):control group (C),hypoxia reoxygenation group (H/R) and five treated groups:SF group(two dose 20 g/kg interval 1 h),DJ-1 group(10 g/kg),SJ-1 group(two dose 10 g/kg interval 1 h),DJ-2 group(20 g/kg) and SJ-2 group(two dose 20 g/kg interval 1 h).After 1 hour of hypoxia followed by 1 h of reoxygenation lactate dehydrogenase leakage(LDH) were analysed.Choosing SJ-2 group,control group,H/R group and Shenfu group to valuate cell viability by MTT and the growth curves were drawn at the time points of 0,24,48,72,96 h to see if there any difference in the groups.Results:There is significant difference in LDH between control group and H/R group(P < 0.01).SJ-1 group is better than H/R group(P < 0.05);SJ-2 group is more better than H/R(P < 0.01);SJ-1 and SJ-2 are better than Shenfu group.In cell viability at the time points of 24 h SJ-2 group is higher than Shenfu group and H/R group.There is no significant difference between control group and SJ-2 group at the time points of 72 h. Conclusion:Astragali liquid have significant protective effect on injury of intestinal epithelial cell induced by hypoxia reoxygenation.