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Flow-injection biamperometric determination of epinephrine
Authors:JV Garcia Mateo  Anatol Kojlo
Institution:1. Departamento de Quimica, Universidad Politecnica de Valencia, Valencia, Spain;2. Departamento de Quimica, Colegio Universitario CEU, Moncada Valencia, Spain;3. Institute of Chemistry, Warsaw University Branch, Al. J. Pi?sudskiego 11/4, 15-443 Bia?ystok, Poland;1. Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, SK-832 32 Bratislava, Slovakia;2. Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, SK-832 32 Bratislava, Slovakia;3. Department of Gastroenterology, St Michael’s Hospital, Satinského 1, SK-811 08 Bratislava, Slovakia;1. Chemical Department, M.V. Lomonosov Moscow State University, Moscow, 119991, Russian Federation;2. National Research Centre “Kurchatov Institute”, Moscow, 123182, Russian Federation;1. Chemical and Environmental Systems Modeling Research Group, National Institute of Fundamental Studies, Kandy, Sri Lanka;2. Korea Biochar Research Center & Department of Biological Environment, Kangwon National University, Chuncheon 200-701, Republic of Korea;3. School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India;1. Department of Pharmaceutical Chemistry, Semmelweis University, Budapest, Hungary;2. Research Group of Drugs of Abuse and Doping Agents, Hungarian Academy of Sciences, Hungary;1. Departamento de Química e Física, Universidade Federal do Espírito Santo, Alto Universitário, sn, 29500-000 Alegre, ES, Brazil;2. Departamento de Química, Universidade de São Paulo, Av. Bandeirantes, 3900, 14040-901 Ribeirão Preto, SP, Brazil
Abstract:A flow-injection manifold is proposed for the determination of epinephrine. The experimental procedure is based on the indirect biamperometric detection of the drug by using Fe(III)-Fe(II) as an indicating redox system and a flow-through detector with two polarized Pt wire electrodes. The calibration graph is linear over the range 0.3–20 μg ml?1 of epinephrine. The relative standard deviation for the determination of 10 μg ml?1 of epinephrine is 1.5% (n = 25) and the sample throughput is 153 h?1. The method was applied to the determination of epinephrine in two commercially available pharmaceutical preparations.
Keywords:Biamperometry  Flow injection  Catecholamines  Epinephrine  Adrenaline
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