| 小鼠血管抑素基因的克隆及真核表达载体构建 |
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| 引用本文： | 李秀娟,杨旭,李治国,李修义. 小鼠血管抑素基因的克隆及真核表达载体构建[J]. 河南大学学报(医学版), 2003, 22(4): 1-2 |
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| 作者姓名： | 李秀娟 杨旭 李治国 李修义 |
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| 作者单位： | 河南大学,医学院,河南,开封,475001;吉林大学,公共卫生学院,吉林,长春,130021 |
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| 基金项目： | 国家自然基金资助项目 (编号 :30 170 2 90 ) |
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| 摘 要： | 目的 :克隆小鼠血管抑素 (Angiostatin)cDNA ,构建其真核表达载体pCMV -Angiostatin重组质粒 ,为进一步研究其抗肿瘤作用奠定基础。方法 :根据Genebank中小鼠血管抑素基因序列 ,用RT -PCR方法从鼠肝脏中扩增出AngiostatincDNA ,连接 pMD18T载体测序 ,经测序证实后 ,通过中间载体 pKS ,构建pCMV -Angiostatin重组质粒。结果 :测序表明扩增的AngiostatincDNA序列与报道基本一致 ,AngiostatincDNA正确插入表达载体。 结论 :成功地克隆小鼠Angiostatin基因并完成其真核表达载体的构建
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| 关 键 词： | 小鼠血管抑素 pCMV-Angiostatin重组质粒 构建 |
| 文章编号： | 1008-2867(2003)04-0001-02 |
| 修稿时间： | 2003-11-27 |
Cloning of Mouse Angiostatin and Construction of its Eukaryotic Expression Vector |
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| LI Xiu-juan ,YANG Xu ,LI Zhi-guo ,LI Xiu-yi. Cloning of Mouse Angiostatin and Construction of its Eukaryotic Expression Vector[J]. Journal of Henan University, 2003, 22(4): 1-2 |
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| Authors: | LI Xiu-juan YANG Xu LI Zhi-guo LI Xiu-yi |
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| Affiliation: | LI Xiu-juan 1,YANG Xu 1,LI Zhi-guo 2,LI Xiu-yi 2 |
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| Abstract: | Objective: To clone the sequence of mouse Angiostatin cDNA and construct its eukaryotic expression vector, provide a base for its antitumor effect. Methods: According to the sequence of Mouse angiostatin cDNA in Genebank, Mouse angiostatin cDNA was amplified by RT-PCR and ligated into pMD18T vector. Through the vector pKS, the pCMV-Angiostatin recombinant plasmid was constructed. Results: The sequence of mouse Angiostatin cDNA is identical with reported. Angiostatin cDNA was inserted into expression vector correctly. Conclusion: Mouse Angiostatin cDNA is cloned and pCMV-Angiostatin recombinant plasmid is completed successfully. |
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| Keywords: | mouse Angiostatin cDNA pCMV-Angiostatin recombinant plasmids construct |
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