| HPLC分析不同来源藏药当佐中4种指标成分的含量 |
| |
| 引用本文： | 李岑,肖远灿,杨红霞,杜玉枝,魏立新. HPLC分析不同来源藏药当佐中4种指标成分的含量[J]. 中国中药杂志, 2011, 36(8): 1046-1049 |
| |
| 作者姓名： | 李岑 肖远灿 杨红霞 杜玉枝 魏立新 |
| |
| 作者单位： | 1. 中国科学院西北高原生物研究所,青海,西宁,810008;青海省藏药药理学和安全性评价研究重点实验室,青海,西宁,810008;中国科学院,研究生院,北京,100049
2. 中国科学院西北高原生物研究所,青海,西宁,810008;青海省藏药药理学和安全性评价研究重点实验室,青海,西宁,810008 |
| |
| 基金项目： | 国家"十一五"科技支撑计划项目(2007BAI48B02) |
| |
| 摘 要： | 目的:建立HPLC同时测定藏药当佐中没食子酸、羟基红花黄色素-A、桂皮醛、胡椒碱含量的方法,对不同来源样品进行测定,以建立当佐的质量控制标准.方法:采用Waters XTerra RP-C18(4.6 mm×250 mm,5 μm)色谱柱,以甲醇-水(含0.1%冰乙酸)为流动相,梯度洗脱(0-22.5 min,流动相比例5:95-50:50;22.5-40 min,流动相比例50:50-80:20),流速为1.0 mL·min-1,检测波长为270 nm.结果:在此色谱条件下,没食子酸、羟基红花黄色素-A、桂皮醛、胡椒碱达到基线分离,且线性良好,线性范围分别为0.040-0.640μg(r=0.999 8),0.090-1.440 μg(r=0.999 9),0.031-0.500μg(r=0.999 9),0.092-1.477μg(r=0.998 9),平均回收率(n=6)分别为97.42%(RSD 1.9%),97.55%(RSD 2.9%).98.69%(RSD 0.96%),96.72%(RSD4.0%);不同来源当佐中该4种成分的含量变化分别为0.113-1.69,0.889-1.51.0.000-0.606,1.96-2.75 mg·g-1.结论:本研究所建立的方法,能够准确快速地测定当佐中指标成分的含量,可为该药的全面质量评价提供参考.
|
| 关 键 词： | HPLC 当佐 没食子酸 羟基红花黄色素-A 桂皮醛 胡椒碱 |
| 收稿时间： | 2010-09-09 |
HPLC determination of four components in Tibetan medicine Dangzuo of different tibetan regions |
| |
| LI Cen,XIAO Yuancan,YANG Hongxi,DU Yuzhi and WEI Lixin. HPLC determination of four components in Tibetan medicine Dangzuo of different tibetan regions[J]. China Journal of Chinese Materia Medica, 2011, 36(8): 1046-1049 |
| |
| Authors: | LI Cen XIAO Yuancan YANG Hongxi DU Yuzhi WEI Lixin |
| |
| Affiliation: | Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810008, China;Qinghai Province Key Laboratory of Tibetan Medicine Pharmacology and Safety Assess, Xining 810008, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China;Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810008, China;Qinghai Province Key Laboratory of Tibetan Medicine Pharmacology and Safety Assess, Xining 810008, China;Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810008, China;Qinghai Province Key Laboratory of Tibetan Medicine Pharmacology and Safety Assess, Xining 810008, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China;Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810008, China;Qinghai Province Key Laboratory of Tibetan Medicine Pharmacology and Safety Assess, Xining 810008, China;Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810008, China;Qinghai Province Key Laboratory of Tibetan Medicine Pharmacology and Safety Assess, Xining 810008, China |
| |
| Abstract: | |
| |
| Keywords: | HPLC Dangzuo gallic acid hydroxysafflor yellow A cinnamic aldehyde piperine |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
|
