Microbial Degradation of Cephalothin by Cephalothin-Susceptible Escherichia coli |
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| Authors: | Tsuneo Nishiura Yukimichi Kawada Yoko Shiomi Koji O''''Hara Megumi Kono |
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| Affiliation: | 1Department of Urology, Gifu University School of Medicine, Gifu 500 Japan;2Department of Microbiology, Tokyo College of Pharmacy, Tokyo 192–03, Japan |
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| Abstract: | Cephalothin (CET)-susceptible Escherichia coli, which can degrade CET after prolonged incubation in broth containing a concentration of the drug greater than the minimum inhibitory concentration, was found in a clinical specimen. The substrate specificity of the partially purified enzyme to cephalosporin analogs strongly indicated the occurrence of CET-specific degradation. Nuclear magnetic resonance analysis of the degradation reaction demonstrated the appearance of two new signals attributed to deacetyl CET. This suggests the possibility of the presence of acylesterase. |
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