心理应激对模拟高原环境下大鼠肺损伤影响的实验研究

目的：探讨心理应激对模拟高原低压低氧环境下大鼠肺损伤的影响。方法：雄性SD大鼠随机分为对照组（Con组）、低氧组（Hy组）、心理应激（2次／d）组（Ps1组）、心理应激（2次／d×2d）组（Ps2组）、心理应激（2次／d）＋低氧组（Ps1＋Hy组）和心理应激（2次／d×2d）＋低氧组（Ps2＋Hy组）。通过测定大鼠右肺湿／干比值、全湿肺／体重比值、右肺含水比值、血管“袖套”面积和血管“袖套”面积／血管面积的比值来评定肺损伤的程度。结果：与Con和Hy组比较，Ps2＋Hy组的右肺湿／干比值、肺湿重／体重比值、血管“袖套”面积和血管“袖套”面积／血管面积比值均显著增高（P分别〈0．01，0．05）。结论：心理应激可加重急性低压低氧环境导致的大鼠肺损伤。

Effect of psychological stress on lung injury induced by simulated acute high-altitude hypobaric hypoxia in rats

Objective：To examine the effect of psychological stress on lung injury induced by simulated acute high- altitude hypobaric hypoxia in rats. Methods： Male SD rats were randomly divided into six groups： control （Con group）, hypoxia （Hy group）, psychological stress （twice daily × 1 day） （Ps1 group）, psychological stress （ twice daily ×2 day） （Ps2 group）, psychological stress （ twice daily × 1 day） ＋ hypoxia （ Ps1＋ Hy group）, and psychological stress （twice daily ×2 day） ＋ hypoxia （Ps2 ＋ Hy group）. To assess the tung injury, the wet/dry weight ratio of fight lung, the wet lung/body weight ratio, the water ratio of fight lung, the perivascular cuff area of small vessels accompanied with bronchiole and the perivascular cuff area/ vessels area ratio were determined. Results ： Compared with the Con and Hy group, the wet/dry weight ratio of right lung , the wet lung/body weight ratio, the perivascular cuff area and the perivascular cuff area/vessels area ratio were significantly higher in Ps2 ＋ Hy groups （ P 〈 0.01, P 〈 0.05 ）. Conclusion： Psychological stress exacerbates the lung injury induced by simulated acute high -altitude hypobaric hypoxia in rats.