孕妇外周血基质金属蛋白酶-9、白介素-6水平联合B超测定宫颈长度预测早产的研究

目的探讨孕妇外周血基质金属蛋白酶一9（MMP-9）、白介素-6（IL-6）及宫颈长度对早产预测的应用价值。方法选择本院正常妊娠组孕妇23例，先兆早产孕妇39例，按是否继续妊娠为2个亚组，早产分娩组18例，继续妊娠组21例，分别采集各组静脉血，分离血浆应用ELISA方法测定MMP-9及IL-6水平，同时采用B超测定宫颈长度，预测孕妇早产发生情况。结果（1）早产分娩组孕妇MMP-9及IL-6水平显著高于正常妊娠组及继续妊娠组（47．24±6．41）ng／ml，（523．24±62．35）pg／mlvs（20．39±7．23）ng／ml，（219．29±80．23）pg／ml，（32．18±5．16）ng／ml，（406．18．4-71．52）pg／ml]，其差异均有统计学意义（P〈0．01），继续妊娠组血浆MMP-9及IL-6水平显著高于正常妊娠组（32．18±5．16）ng／ml，（406．18±71．52）pg／mlvs（20．39±7．32）ng／ml，（219．29±80．23）pg／ml]，其差异均有统计学意义（P〈0．01）；（2）早产分娩组宫颈长度显著低于正常妊娠组及继续妊娠组（15．62±2．31）mmVS（31．05±1．48）mm，（26．14±3．12）mm，P〈0．01]，继续妊娠组孕妇宫颈长度稍低于正常妊娠组（26．14±3．12）mmVS（31．05±1．48）mm]，但差异无统计学意义（P〉0．05）。结论孕妇外周血中MMP-9、IL-6水平高低及宫颈长度可作为早产发生的预测指标。

Evaluation of plasma matrix metalloproteinase-9 and Interleukin-6 combined with cervical length in the prediction of premature delivery

Objective To investigate predictive value of matrix metalloproteinase-9 （MMP-9） and Interleukin-6 （IL-6） combined with cervical length in premature delivery. Methods Plasma samples of 62 pregnant women were detected in this study. Samples were divided into three groups based on pregnancy outcomes. MMP-9 and IL-6 values were determined by ELISA. Cervical length was measured by transvagi- nal sonography. Results The level of MMP-9 of the pregnant women in the premature delivery group was significantly higher than that of continuous pregnant group and that of normal pregnant women （47. 24 ± 6.41 ） ng/ml, （523.24 ± 62. 35 ） pg/ml vs （20. 39 ± 7.23 ） ng/ml, （219. 29 ± 80. 23 ） pg/ml, （ 32. 18 ± 5. 16）ng/ml, （406. 18 ±71.52）pg/ml, P 〈0. 01 ]. The level of MMP-9 and IL-6 of the pregnant women in the continuous pregnant group was significantly higher than that of normal pregnant women （ 32. 18 ± 5.16） ng/ml, （406. 18 ± 71.52） pg/ml vs （20. 39 ± 7. 32）ng/ml, （219. 29 ±80. 23 ） pg/ml, P 〈 0. 01 ]. Cervical length of the pregnant women in the premature delivery group was significantly shorter than that of normal pregnant women and that of continuous pregnant group （ 15.62 ± 2. 31 ） mm vs （ 31.05 ± 1.48 ） mm, （26. 14±3.12）mm, P 〈0. 01 ]. Conclusions Detection of level of MMP-9 and IL-6 combined with measurement of cervical length can be used as predicting index of premature delivery.