基质金属蛋白酶11和12在子宫颈癌中的表达及相关性研究

目的通过检测MMP-11、MMP．12在宫颈癌组织中的表达，探讨其与宫颈癌临床病理的关系，为诊治提供理论依据。方法采用免疫组化方法检测20例正常宫颈组织、36例宫颈上皮内瘤变组织（CIN）以及80例宫颈癌组织中MMP-11、MMP-12的表达情况，分析各组宫颈组织中的阳性表达与临床病理的关系。结果（1）MMP-11及MMP-12在宫颈癌组织中的阳性表达率（82．50％，81．25％）高于宫颈非典型增生组织（69．44％，61．11％）和正常宫颈组织（5．00％，0，P〈0．05）。（2）MMP．11及MMP-12的阳性表达与宫颈癌的组织学分级、临床分期、间质浸润深度、有无脉管入侵、有无淋巴结转移有关（P〈0．05）。（3）宫颈癌组织中MMP-11与MMP-12的阳性表达呈正相关（L=0．706，P〈0．05）。结论MMP-11及MMP-12在宫颈癌组织呈高表达，二者阳性表达在宫颈癌的浸润转移过程中起重要作用；联合检测MMP-11及MMP-12的表达可能对宫颈癌的早期诊断、判断肿瘤恶性程度及预后具有参考意义。

The expression and relationship of metalloproteinases 11 and 12 in cervical cancer

Objective This study was aimed to determine the expression of matrix metalloprotein- ase-11 （ MMP-11 ） and MMP-12 in cervical cancer and the relationship between their expression with clinico- pathological characteristics and the potential use as a prognostic and therapeutic agent. Methods Strepta- vidin-biotin-protein-linked peroxidase method（ ie, SP） immunohistochemical method was used to detect the expression of MMP-11, MMP-12 in 20 cases of normal cervical tissue,36 cases of CIN tissue,80 cases of cervical cancer. MMP-11 and 12 expression in cervical cancer and clinicopathological parameters of rele- vance were analyzed, and the relationship between MMP-11 ,-12 was studied in the development of cervical cancer for a preliminary study. Results The positive expression rate （82. 50% ,81.25 % ）of MMP-11 and- 12 in cervical cancer was significantly higher than that of cervical dysplasia （CIN） specimens （69.44% , 61.11% ） and normal cervical tissues （5.00% ,0） , the difference was statistically significant （ P 〈 O. 05 ）. （2）The expression of MMP-11 and-12 in cervical histological grade, clinical depth of stromal infiltration, with or without vascular invasion, with or without lymph stage, node metastasis was different. The difference was statistically significant （ P 〈 0. 05）. （3）MMP-11 and-12 expression was significantly correlated. Con- clusions The expression of MMP-11 and-12 in cervical cancer tissues prompts that MMP-11 and-12 plays a central role in the course of invasion and metastasis of cervical carcinoma. MMP-11 and-12 joint detection may have a reference value in terms of the inchoate diagnosis of the cervical cancer, the evaluation and prognosis of tumor malignances degree.