干细胞特异性标志物Oct-4在表皮肿瘤中表达的临床实验研究

目的探讨表皮非黑素肿瘤中干细胞特异性标志物Oct-4的表达情况以及在表皮肿瘤发生中的作用。方法本研究选择20例人皮肤鳞状细胞癌（scc）、20例基底细胞癌（BCC）、20例脂溢性角化（SK）和20例正常人皮肤组织，采用EnVision免疫组化检测各组组织中干细胞特异性标志物Oct-4和增殖细胞核抗原（PCNA）的表达情况，分析其表达意义。结果（1）Oct-4在皮肤SCC、BCC中表达强度分别为90％（18／20）、80％（16／20），与正常皮肤组织65％（13／20）和皮肤SK75％（15／20）相比明显增高（P〈0．05），Oct-4在皮肤SCC和BCC之间的表达比较差异无统计学意义（P〉0．05）。Oct-4在皮肤SK和正常皮肤组织之间的表达比较差异无统计学意义（P〉0．05）。Oct-4在皮肤SK和BCC之间的表达比较差异无统计学意义（P〉0．05）。（2）PCNA在皮肤SCC、BCC中表达强度分别为95％（19／20）、90％（18／20），与正常皮肤组织75％（12／20）和皮肤SK85％（17／20）比较差异有统计学意义（P〈0．01）；在皮肤SCC和BCC之间的表达比较差异有统计学意义（P〈0．05）；在皮肤SK和正常皮肤组织之间的表达比较差异有统计学意义（P〈0．05）；而PCNA在皮肤SK和BCC的表达比较差异无统计学意义（P〉0．05）。（3）Oct-4在皮肤SCC、BCC中表达强度与细胞周期蛋白PCNA表达阳性强度均呈正相关（-=0．450，0．457，P〈0．05）。结论干细胞特异性标志物Oct-4在BCC和SCC中表达强度明显增高，可能与BCC和SCC发生发展有关。PCNA在BCC和SCC中强阳性表达，可能参与了BCC和SCC增殖过程。Oct-4和PCNA在皮肤SCC、BCC中的阳性表达强度呈正相关性，Oct-4可能通过影响细胞增殖参与了BCC和SCC的发病过程。

Oct-4 expression in epidermal tumors

Objective The aim of this study was to understand the role of specific markers of stem cells Oct-4 expression in the development of human epidermal non-melanoma cutaneous tumors. Methods The paraffin-embedded samples were retrieved from files of pathology department at our hospital, including 20 cases of skin squamous cell carcinomas （ SCC）, 20 cases of basal cell carcinomas （ BCC）, 20 cases of seborrhoeic keratosis （SK） and 20 cases of normal skin （from head, face, trunk, extremities）. The ex- pression of Oct-4 and PCNA were observed by immunohistochemical staining technique. Results Oct-4 protein was abnormally increased in SCC and BCC compared to normal skin and SK （ P 〈 0. 05 ）. However there were no significant difference of Oct-4 protein expression between SCC and BCC （ P 〉 0. 05 ）. There were also no significantly different Oet-~ protein expression between Sk and the normal skin （ P 〉O. 05 ） , and no significantly different Oct-4 protein expression between SK and BCC （ P 〉 0. 05）. PCNA protein was abnormally increased in SCC and BCC compared to normal skin and SK （ P 〈 0.01 ）. There were significantly different PCNA protein expression between SCC and BCC（ P 〈0. 05）. There were also significantly different PCNA protein expression between SK and the normal skin （ P 〈 0. 05）. However there were no significant difference of PCNA protein expression between SK and BCC （ P 〉 0. 05 ）. There were positive correlation between the expression intensity of Oct-4 and PCNA in SCC and BCC. Conclusions The abnormal expression of Oct-4 may have an important role in the development of BCC and SCC. Positive Oct-4 expression cells may be the tumor stem cell in SCC and BCC. There were positive correlation between the expression intensity of Oct-4 and PCNA in SCC and BCC. The over expression Oct-4 in BCC and SCC may play an important role in proliferation of tumor.