二甲双胍对高脂环境下大鼠成肌细胞糖代谢的影响

目的探讨二甲双胍对经棕榈酸诱导后脂肪变性的大鼠成肌细胞糖代谢的影响。方法取Wister大鼠乳鼠骨骼肌细胞进行原代细胞培养，取第5代成肌细胞，对照组用含0．5％BSA的DMEM培养12h；高脂组用0．3mmol／LPA（含0．5％BSA）培养12h；干预组分为3组，分别在高脂干预同时分别加入2．5、5．0和7．5μg/ml的二甲双胍，继续培养24h，用同位素示踪法检测葡萄糖摄取。结果与对照组相比，高脂组葡萄糖摄取明显下降（1．83士0．34）10^-2pmd／（min·g）vs（2．33±0．47）10-2pmd／（min·g），P〈0．05]。在高脂组中随着给予二甲双胍的浓度逐渐增加，葡萄糖摄取逐渐增加，并呈剂量反应关系，其中7．5μg／ml的二甲双胍糖摄取量最多（2．31±0．29）10-2pmd／（min·g）]。结论二甲双胍可增加高脂环境下大鼠成肌细胞的糖摄取，并且存在剂量依赖关系。

The effect of metformin on glucose metabolism of rat myoblast with high fat environment

Objective To explore the effect of metformin on glucose metabolism of rat myoblast with high fat environment. Methods The skeletal muscles of suckling Wister rats were subjected to primary culture. The myoblasts of passage 5 were divided into 5 groups ： The control group was cultured in DMEM with 0. 5% BSA for 12 h. The high-fat group was exposed to 0. 3 mmol/L palmitic acid for 12 h. On the basis of high - fat treatment, three intervention groups were added with 2. 5,5.0 and 7.5 μg/ml mefformin, respectively, for additional 24 h. Isotope tracer method was conducted to show the influence on 2-dexoy-D- 3H]glucose uptake of rat myoblast. Results With the increasing concentration of metformin, 3H]-G uptake elevated （ 1.83 ±0. 34） 10-2pmd/（min·g） vs （2. 33±0. 47） 10-2pmd/（min·g）, P 〈 0.05 ], and the group of 7. 5μg/ml metformin uptakes the most. Conclusions Mefformin can upregulate the glucose-uptake in rat myoblasts under the environment of high fat, and it shows a dose - dependent relationship.