HPV感染及负荷量对宫颈癌前病变及宫颈癌的作用研究

目的探讨人乳头瘤病毒感染（HPV）及负荷量对宫颈癌前病变及宫颈癌的作用。方法选择2009年3月-2012年3月在新疆医科大学附属肿瘤医院经病理检查确诊的宫颈病变患者1326例为研究对象，其中慢性宫颈炎1000例，CINI52例，CINⅡ40例，CINII142例，宫颈癌92例，采用杂交捕获二代（HC-Ⅱ）方法定量检测宫颈HPV-DNA的含量。结果（1）慢性宫颈炎组、CIN组、宫颈癌组HPV～DNA阳性率分别为13．5％、67．9％、94．5％，各组阳性率随着宫颈病变的程度加重而增加（P〈0．001）。（2）CINI组、CINⅡ组、CNIII组中HPV-DNA阳性率分别为46．2％、57．5％、78．8％，各组阳性率随着CIN级别增高而增高（P〈0．001）。（3）在HPV—DNA负荷量〉500时，HPV—DNA病毒负荷量随着宫颈病变程度的加重而增加，组间比较差异有统计学意义（P〈0．001）。结论HPV-DNA病毒感染及其负荷量与宫颈癌前病变及宫颈癌发生有关；HC-Ⅱ检测对预测CIN、宫颈癌发展有重要意义。

The study into the loads of HPV and infection of HPV with a virus incervical carcinom and cervical intraepithelial neplasia

Objective To explore HPV infection and loads on cervical carcinoma and Cervical Intraepithelial neoplasia. Methods 1 326 cervical cancer sufferers were confirmed by pathological examina- tion from march 2009 to march 2012 and were taken as subjects, including 1 000 chronic cervicitis cases, 52 CINI cases, 40 CIN eases, 142 CINIII eases, 92 cervical cancer cases. Results （1） The rate of HR- HPV infection was 13.5~//00 in cervicitis, 67.9% in CIN, 94.5% in Cervical Cancer, each positive rate in- creased as the degree of cervical lesion increases （P ~0.001）; （2） The rate of HR-HPV infection was 46.2G in CIN I , 57.5G in CIN 11 , 78.8G in CIN HI, each positive rate increased as the CIN level increased （P ~ 0.001）~ （3） The comparison among four groups show that the distribution of HPV quantity was significantly different if HPV-DNA〉500 （P 〈0.001）. Conclusion The viral loads of HPV DNA have a correlation with the grade of Cervial Cancer, HC-Ⅱ test has important significance in CIN and the cervical cancer screening.