葛根素对大鼠肝缺血再灌注损伤的保护作用

目的:观察葛根素(Pue)对肝脏缺血再灌注损伤的保护作用.方法:建立大鼠全肝缺血再灌注模型,比较Pue处理对再灌注血ET-1、NO和ALT水平,肝组织中MDA、细胞总钙含量和SOD活性,以及肝组织光镜下的形态学改变.结果:Pue处理可使血NO含量、肝组织SOD活性明显增高;而血ET-1、ALT含量及肝组织MDA、细胞总钙含量明显降低.肝组织形态损伤也明显减轻.Pue保护效应与剂量大小明显相关.结论:葛根素对肝缺血再灌注损伤有显著的药物预适应作用,其主要机制是清除氧自由基、减轻细胞内Ca 超载以及改善微循环.

The protective effect of puerarin on hepatic injury induced by ischemia/reperfusion in rat

Objective: To evaluate the protective effects of puerarin on hepatic injury during ischemia/reperfusion procedure, and to explore the pharmacological mechanism of puerarin. Methods: 40 SD rats were divided randomly into 4 groups (n=10): I/R groups (A), puerarin group with low dose (20mg/kg) (B1), puerarin group with moderate dose (40mg/kg) (B2), puerarin group with high dose (80mg/kg) (B3). Hepatic ischemia reperfusion models were setted up. At the end of reperfusion, the NOx?ET?ALT levels in plasma of each group were detected, as well as the SOD?MDA and Ca levels in tissue were observed. Results: In puerarin groups the serum level of NO were higher than that in I/R group (P<0.01); while plasma levels of ET?ALT were lower (P<0.01). The SOD level of hepatic tissue were higher in puerarin groups than that in I/R group (P<0.01); as well as MDA?Ca levels were lower (P<0.01). All these changes were related to the dose of the puerarin. And abnormal morphological changes of liver during I/R procedure were ameliorated remarkably in puerarin groups. Conclusions: Puerarin attenuates the hepatic injury of ischemia/reperfusion by inhibiting the production of oxygenic free radicals; preventing hepatocellular Ca overload and improving the function of microcirculation. And the protective effect was related to the dose of the puerarin.