基于正常小鼠排便作用的益智仁燥性效应表征研究

目的: 观察益智仁对正常小鼠排便作用的影响及作用机制，从而对益智仁的燥性效应进行表征。 方法: 将SPF小鼠分为正常动物组、益智仁20，10，5 g·kg^-1剂量组及益智仁20 g·kg^-1与增液汤20 g·kg^-1联用组，各组动物每天灌胃给药1次，连续28 d，在第7，14，21，28 d观察对动物排便作用的影响，并从肠道水分、结肠肌电方面阐明益智仁燥性对肠道影响的机制。 结果: 在连续6 h观察中，益智仁低剂量组在28 d的首次排黑粪时间为142.7 min，干粪粒数为10.1粒，干粪重量为176.6 mg;中剂量组在21 d的首次排黑粪时间为145.4 min，干粪粒数为10.2粒，干粪重量为177.0 mg;高剂量组在21 d的首次排黑粪时间为160.3 min，干粪粒数为9.7粒，干粪重量为170.5 mg，说明益智仁能呈剂量依赖和时间依赖使得正常动物逐渐出现便秘症状。在28 d时，益智仁低、中、高剂量组的大肠肠腔水分含量分别是66.23%，64.42%，61.53%，均能显著减少大肠肠腔水分含量。在28 d时，益智仁低、中、高剂量组的结肠肌电频率分别是43.40，45.63，48.26次/min，结肠肌电振幅分别是66.23，64.42，61.53 mV，能显著增快结肠肌电频率及降低结肠肌电振幅。增液汤对于益智仁引起的上述便秘作用有显著的拮抗作用。 结论: 益智仁燥性效应表征能引起正常小鼠的便秘作用，且与促进肠道水分重吸收和减弱肠道传输能力有关。

Study of Dry Effect Characterization of Alpiniae Oxyphyllae Fructus by Defecating Function of Normal Mice

Objective: To study the effect of Alpiniae Oxyphyllae Fructus on defecating function of normal mice and its possible mechanism, and the dry effects were characterized. Method: The SPF mice were divided into 5 groups: normal group, Alpiniae Oxyphyllae Fructus with different doses groups (20,10,5 g· kg^-1), Alpiniae Oxyphyllae Fructus(20 g· kg^-1) and increase liquid soup(20 g·kg^-1) combination group. All mice were orally administered once a day for 28 d. In the 7,14,21,28 d to observe the effects of defecation function to animals, and from the aspects of intestinal moisture, colonic electromyography to explain the mechanism that the dry effects of Alpiniae Oxyphyllae Fructus impacting on gut. Result: After 6 h observation, the first time of defecation with low dose was 142.7 min, dry manure was 10.1 grains, weight was 176.6 mg in 28 d; the first time of defecation with moderate dose was 145.4 min, dry manure was 10.2 grains, weight was 177.0 mg in 21 d; the first time of defecation with high dose was 160.3 min, dry manure was 9.7 grains, weight was 170.5 mg in 21 d. Alpiniae Oxyphyllae Fructus is able to make the normal animal appear symptoms of constipation gradually through dose-dependent and time-dependent. After administrated for 28 days, the moisture content of colon lumen of low dose, moderate dose and high dose group of Alpiniae Oxyphyllae Fructus was 66.23%,64.42%,61.53%, significantly reduce the moisture content of colon lumen; frequency of colonic electromyography of low dose, moderate dose and high dose group of Alpiniae Oxyphyllae Fructus was 43.40,45.63,48.26 times per minute, the amplitude of colonic electromyography was 66.23,64.42,61.53 mv, significantly faster frequency of colonic electromyography, reduce the amplitude of colonic electromyography.Increase liquid soup have obvious antagonism effects on above-mentioned constipation caused by Alpiniae Oxyphyllae Fructus. Conclusion: Dry effect characterization of Alpiniae Oxyphyllae Fructus can cause constipation of normal mice, and it's related to the promotion of intestinal water reabsorption and reduced intestinal transit capacity.