| hTERT基因反义核酸对Hela宫颈癌细胞端粒酶活性的影响 |
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| 引用本文： | 韩风华,辛晓燕,杜辉,王健.hTERT基因反义核酸对Hela宫颈癌细胞端粒酶活性的影响[J].西南国防医药,2002,12(1):7-10. |
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| 作者姓名： | 韩风华 辛晓燕 杜辉 王健 |
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| 作者单位： | 第四军医大学西京医院,西安,710032 |
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| 摘 要： | 研究人类端粒酶催化亚单位（hTERT)基因的反义寡核苷酸对宫颈癌细胞的影响。方法：采用TRAP－ELISA检测Hela细胞在反义核酸处理前后端粒酶活性的变化。结果：hTERT基因反义核酸能有效抑制Hela细胞端粒酶活性。结论：hTERT基因反义核酸能显著抑制肿瘤细胞的端粒酶活性，为肿瘤治疗提供了一个有效的新途径。
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| 关 键 词： | hTERT基因 反义核酸 Hela 宫颈癌 细胞 端粒酶 活性 端粒酶催化亚单位 反义治疗 |
ATTENUATION OF TELOMERASE ACTIVITY BY USING AN ANTISENSE OLIGONUCLEOTIDE AGAINST hTERT mRNA IN CERVICAL CANCER CELLS. |
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| Han Fenghua,Xin Xiaoyan,Du Hui,Wang Jian.ATTENUATION OF TELOMERASE ACTIVITY BY USING AN ANTISENSE OLIGONUCLEOTIDE AGAINST hTERT mRNA IN CERVICAL CANCER CELLS.[J].Medical Journal of National Defending forces in Southwest China,2002,12(1):7-10. |
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| Authors: | Han Fenghua Xin Xiaoyan Du Hui Wang Jian |
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| Institution: | Han Fenghua,Xin Xiaoyan,Du Hui,Wang Jian Department of Obstetrics and Gynecology,Xijing Hospital,Fourth Military Medical University,Xi'an,710032 |
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| Abstract: | Objective: To study the effects of antisense oligonucleotide against human telomerase catalytic subunit mRNA(hTERT mRNA)on human telomerase activity in cervical cancer cells in vitro. Method :Telomerase activity of cultured cervical cancer cells(Hela cells) was measured using the TRAP-ELISA assay before and after antisense treatment. Result: The antisense treatment could effectively inhibite the telomerase activity of Hela cells. Conclusion: Antisense oligonucleotide direct against hTERT mRNA could effectively inhibite the telomerase activity of tumor cells, which show an effective and novel approach for treatment of cancers. |
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| Keywords: | telomerase telomerase catalytic subunit cervical carcinoma antisense treatment |
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