| 重楼活性单体PP-22对人结肠癌SW620细胞增殖和凋亡的影响 |
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| 引用本文： | 肖晓慧,宫瑞松,张相强,李振华,代立婷,蒋建伟.重楼活性单体PP-22对人结肠癌SW620细胞增殖和凋亡的影响[J].中国现代应用药学,2015,32(10):1175-1180. |
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| 作者姓名： | 肖晓慧 宫瑞松 张相强 李振华 代立婷 蒋建伟 |
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| 作者单位： | 暨南大学医学院临床医学系，广州 510632,暨南大学医学院临床医学系，广州 510632,暨南大学医学院生物化学系，广州 510632,暨南大学医学院生物化学系，广州 510632,暨南大学医学院生物化学系，广州 510632,暨南大学医学院生物化学系，广州 510632 |
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| 基金项目： | 广东省科技计划项目(2011B031800012)；广东省自然科学基金(2014A030313356)；暨南大学第一临床医学院科研培育基金青年项目(2014203) |
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| 摘 要： | 目的 探讨重楼活性单体PP-22对人结肠癌SW620细胞增殖和凋亡的影响及其联合5-氟尿嘧啶(5-FU)和洛铂的抗肿瘤效果。方法 采用噻唑蓝(MTT)法和克隆形成抑制实验观察不同浓度PP-22对人结肠癌SW620细胞增殖的抑制作用；分别观察PP-22联合5-FU和洛铂对人结肠癌SW620细胞增殖的抑制作用；碘化丙锭单染流式细胞术检测细胞周期变化及细胞凋亡水平；Western blot法检测PP-22作用后细胞凋亡相关蛋白的表达。结果 重楼单体PP-22能显著抑制SW620细胞的生长，作用呈剂量-效应关系；随着PP-22浓度的增加，细胞克隆形成逐渐减少，与细胞对照组和DMSO组相比有显著差异；PP-22联合不同浓度的5-FU和洛铂作用于SW620细胞48 h，可提高SW620细胞对5-FU和洛铂的敏感性；不同浓度PP-22作用于SW620细胞后，细胞周期阻滞于S期；PP-22作用后存在Caspase-3和Caspase-9蛋白的活化和降解，抗凋亡蛋白Bcl-2、Bcl-xL减少，促凋亡蛋白Bax的表达增加。结论 PP-22能显著抑制人结肠癌SW620细胞增殖，诱导细胞凋亡，提高SW620细胞对5-FU和洛铂的敏感性。
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| 关 键 词： | 重楼 人结肠癌SW620细胞 增殖 凋亡 敏感性 |
| 收稿时间： | 5/5/2015 12:00:00 AM |
| 修稿时间： | 2015/9/27 0:00:00 |
Effects of Active Monomer PP-22 Purified from Paridis Rhizoma on Proliferation and Apoptosis of Human Colorectal Cancer SW620 Cells |
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| XIAO Xiaohui,GONG Ruisong,ZHANG Xiangqiang,LI Zhenhu,DAI Liting and JIANG Jianwei.Effects of Active Monomer PP-22 Purified from Paridis Rhizoma on Proliferation and Apoptosis of Human Colorectal Cancer SW620 Cells[J].The Chinese Journal of Modern Applied Pharmacy,2015,32(10):1175-1180. |
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| Authors: | XIAO Xiaohui GONG Ruisong ZHANG Xiangqiang LI Zhenhu DAI Liting and JIANG Jianwei |
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| Institution: | Department of Clinical Medicine, Medical College, Guangzhou 510632, China,Department of Clinical Medicine, Medical College, Guangzhou 510632, China,Department of Biochemistry, Medical College, Jinan University, Guangzhou 510632, China,Department of Biochemistry, Medical College, Jinan University, Guangzhou 510632, China,Department of Biochemistry, Medical College, Jinan University, Guangzhou 510632, China and Department of Biochemistry, Medical College, Jinan University, Guangzhou 510632, China |
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| Abstract: | OBJECTIVE To investigate the effects of active monomer PP-22 purified from Paridis Rhizoma on proliferation and apoptosis of human colorectal cancer SW620 cells, and explore the antitumous effect of PP-22 combined with 5-fluorouracil(5-FU) or lobaplatin. METHODS MTT assay and cell colone formation inhibitory assay were used to determine the inhibitory effect of PP-22 on human colorectal cancer SW620 cells. The inhibitory effect of PP-22 combined with 5-FU or lobaplatin were observed, respectively. The percentage of apoptotic cells and cell cycle distribution were determined by propidium iodide(PI) single staining flow cytometry. Western blot was used to determine the effect of PP-22 on the expression of apoptosis related proteins. RESULTS MTT assay showed that PP-22 inhibited colorectal cancer SW620 cells proliferation in dose-dependent manner. Cell colony formation inhibitory assay demonstrated that cell clones decreased with the increase of drug concentrations. Compared with PP-22 group, the chemosensitivity of PP-22 combined with 5-FU or lobaplatin was increased significantly. PI staining analysis showed that cell cycle was arrested at S phase. Western blot detecting showed that Caspase-3 and Caspase-9 were activated and degraded. The expression of Bcl-2 and Bcl-xL were downregulated and pro-apoptotic protein Bax was upregulated. CONCLUSION PP-22 inhibits the proliferation and induces apoptosis of SW620 cells. PP-22 also improves the sensitivity of SW620 cells on 5-FU and lobaplatin. |
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| Keywords: | Paridis Rhizoma human colorectal cancer SW620 cells proliferation apoptosis sensitivity |
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