小鼠超排卵胚胎的着床与Lif基因表达水平的关系

目的检测胚胎移植时子宫内膜中自血病抑制因子（1eukemia inhibitory factor，Lif）基因的表达水平，探讨超排卵对小鼠胚胎着床潜能的影响。方法建立超排周期胚胎和自然周期胚胎受体妊娠小鼠模型，比较妊娠率、胚胎着床率与可基因表达水平之间的关系。结果超排周期受体组的妊娠率（20．00％）和胚胎着床率（8．33％）显著低于自然周期组的妊娠率（55．00％）和胚胎着床率（35．00％）。自然周期胚胎和超排周期胚胎受体组内膜中Lif矿基因表达水平相似，妊娠受体组Lif矿基因表达水平显著高于未孕受体组，但单胎妊娠和多胎妊娠受体组内膜中Lif矿基因表达水平相似。结论超排卵可能因影响胚胎的质量而降低具有相同Lif mRNA表达水平的受体鼠的妊娠率和胚胎着床率。

The relationship between the implantation of ovarian stimulated embryos and the expression of leukemia inhibitory factor mRNA in mice metrium

Objective To check the expression of leukemia inhibitory factor (Lif) mRNA, and to study the impact of ovarian stimulation on the ability of embryo implantation in mice. Methods Pregnancy models of mice were established. The relationship between the implantation of ovarian stimulated embryos and the expression of Lif mRNA in mice metrium was analyzed. Results The group of recipients which the transfered embryos were from stimulated cycles had lower pregnancy and implantation rate compared with the group of recipients which the transfered embryos were from non-stimulated cycles (20.00%, 8.33% vs 55.00%, 35.00%). The Lif mRNA expression was similar in the groups of recipients which the transfered embryos were from stimulated and non-stimulated cycles, so was in the groups of recipients which had single or more than one baby, but higher in the group of pregnancy recipients than in the group of unpregnancy recipients. Conclusion Ovarian stimulation may reduce the ability of embryo implantation in mice. Lif mRNA expression is related to the implantation, but not parallel to the number of implantation.