| 人降钙素cDNA的化学合成与克隆 |
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| 引用本文： | 蔡在龙 窦鸿. 人降钙素cDNA的化学合成与克隆[J]. 第二军医大学学报, 1996, 17(5): 447-449 |
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| 作者姓名： | 蔡在龙 窦鸿 |
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| 作者单位： | 第二军医大学基础医学部生物化学教研室 |
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| 摘 要： | 目的；克隆人降钙素ｃＤＮＡ。方法和结果；应用ＡＢＩ３９１ＤＮＡ合成仪，将人降钙素ｃＤＮＡ分成６个片段合成。退火、磷酸化，连接，最终将人降钙素ｃＤＮＡ克隆于载体ｐＧＥＭ７Ｚ（＋），经ＤＮＡ双链测离，证明克隆利的人降钙素ｃＤＮＡ序列与设计的完全一致。结论；本研究为寡肽ｃＤＮＡ的化学合成与克隆提供了简捷、可靠的方法；为人的降钙素ＤＮＡ的主效表达奠定了基础。
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| 关 键 词： | 降钙素 cDNA 化学合成 克隆 |
Chemical synthesis and cloning of human calcitonin cDNA |
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| Cai Zailong, Dou Hong, Mao Jifang, Zou Lufeng, Wu Jun, Wang Guanjiang. Chemical synthesis and cloning of human calcitonin cDNA[J]. Former Academic Journal of Second Military Medical University, 1996, 17(5): 447-449 |
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| Authors: | Cai Zailong Dou Hong Mao Jifang Zou Lufeng Wu Jun Wang Guanjiang |
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| Abstract: | Objective: Chemical synthesis and cloning of human calcitonin (hCT) cDNA. Methods and Results: A cDNA coding for human calcitonin was divided into six fragments,synthesized by ABI 391 DNA synthesis instruments and cloned into pGEM7Z(+) vector following phosphorylation, annealing and ligation of six fragments and the sequence of cloned hCT cDNA was proved to be the same as designed. Conclusion: A reliable and forthright method of chemical synthesis of oligo-peptide cDNA (or gene)and cloning is provided in this study. This paper is a basic work for expression of hCT cDNA. |
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| Keywords: | human calcitonin cDNA chemical synthesis clone |
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