槲皮素等对烫伤后小鼠肠粘膜损伤的保护作用

目的　研究槲皮素、芸香苷、黄色素母酮 3种黄酮类化合物对烫伤所致小鼠肠粘膜损伤的保护作用。方法　观察小肠肠粘膜损伤指数的变化 ,检测肠粘膜中蛋白质、DNA含量变化 ,DTNB法测定肠粘膜中GSH的含量 ,并应用DNA解旋的荧光检测法测定DNA损伤程度。结果　槲皮素等三种黄酮类化合物连续 3d灌胃可不同程度的改善烫伤所致肠粘膜损伤状况 ,使肠粘膜损伤指数降低 ,蛋白质、DNA、GSH含量增加 ,DNA损伤程度减轻。其中尤以槲皮素作用较为明显。结论　槲皮素、芸香苷、黄色素母酮 3种黄酮类化合物可明显改善烫伤所致小鼠肠粘膜损伤 ,其机制可能与其抗氧化作用有关。

The protective effects of quercetin et al on the intestinal mucosal injury induced by burn in mice

AIM To investigate the protective effects of three flavonoids quercetin,rutoside and flavone on the intestinal mucosal injury induced by burn in mice. METHOD The change of injury index of small intestine was assayed and the content of protein and DNA were measured. DTNB method were adopted to measure the GSH content in the intestinal mucosa. The level of DNA damaged were determined by measuring the fluorescence. RESULTS The condition of injury of intestinal mucosa were improved in varying degrees after these favonoids were given introgastrically for 3 days. The injury index of intestinal mucosa decreased and the content of protein,DNA and GSH increased. The degrees of DNA damage were reduced also.Quercetin was the most effective one among these three flavonoids. CONCLUSION The flavonoids of quercetin, rutoside and flavone have obvious protective effects on burn induced intestinal mucosal injury, and the mechanism may be related to its antioxydation function.