添加短链脂肪酸的TPN对术后化疗大鼠结肠粘膜细胞增殖作用的研究

目的探讨添加短链脂肪酸(SCFAs)的全肠外营养(TPN)对术后化疗大鼠结肠粘膜细胞增殖作用的影响。方法30只SD大鼠在无渣饮食2d后建立TPN和结肠吻合模型。术后随机分为TPN对照组(对照组)、化疗+TPN组(化疗组)、化疗+TPN+SCFAs组(SCFAs组),每组10只。进行实验5 d,术后第6 d留取标本采用流式细胞术分析结肠粘膜细胞周期,观察其增殖情况。结果化疗组结肠粘膜细胞增殖期百分比(6.23±2.75)%显著低于对照组[(9.81±2.78)%,P=0.014]和SCFAs组[(11.57±3.54)%,P=0.001];化疗组结肠粘膜细胞的增殖指数(11.87±2.61)%显著低于其他2组[(20.25±7.22)%,P=0.002;(19.70±5.12)%,P=0.003]。结论添加SCFAs的TPN能促进术后化疗大鼠结肠粘膜细胞的增殖。

THE EFFECT OF TOTAL PARENTERAL NUTRITION SUPPLEMENTED WITH SHORT-CHAIN FATTYACIDS OF THE PROLIFERATION OF COLON MUCOSAL CELLS IN RATS WITH POSTOPERATIVE CHEMOTHERAPY

Objective To study the effect of total parenteral nutrition supplemented with short-chain fatty acids of the proliferation of colon mucosal cells of rats with postoperative chemotherapy.Methods After 2 days′ clean liquid diet,central venous catheter was placed under general anesthesia and colon anastomosis was performed in 30 SD rats.Thirty rats were randomly divided into three groups of 10.The three groups are: TPN group(control group),5-Fu+TPN group(chemotherapy group) and 5-Fu+TPN+SCFAs group(SCFAs group).On 6th day after operation,samples were taken to detect the cycle of colon mucosal cells through flow cytometry.Results In the chemotherapy group,the percentage of colon mucosal cells at the proliferative stage(Stage S)(6.23±2.75)% was significantly lower than that of the control group [(9.81±2.78)%,P=0.014] and the SCFAs group [(11.57±3.54)%,P=0.001].The proliferative index(PI) of the colon mucosal cells in the chemotherapy group was remarkably lower than that of the control group [(20.25±7.22)%,P=0.002] and the SCFAs group [(19.70±5.12)%,P=0.003].Conclusion TPN supplemented with SCFAs promotes the proliferation of colon enterocyte in postoperative chemotherapy rats.