| 同种异体椎间盘移植的实验研究 |
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| 引用本文： | 阮狄克,沈根标.同种异体椎间盘移植的实验研究[J].中华骨科杂志,1996,16(8):511-515. |
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| 作者姓名： | 阮狄克 沈根标 |
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| 作者单位： | 第四军医大学唐都医院骨科，香港大学骨科学系，第四军医大学病理学教研室 |
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| 摘 要： | 本研究是在自体椎间盘移植实验研究的基础上进一步通过Ｘ线、组织病理、生物学活性、生物化学和生物力学探索异体椎间盘移植是否可存活、功能及其归宿。１２只猴随机分为４组，移植术后３、６、９和１２个月分别处死检测。结果表明移植间盘高度术后下降，但１２个月时仍保持正常高度的６１．４％。光镜下未见明显排斥反应，终板和纤维环结构无明显改变，术后早期可见移植间盘终板软骨增生现象，髓核基质密度增大，成软骨样纤维细胞增生明显。３Ｈ－ｐｒｏｌｉｎｅ掺入较对照组明显增加。术后移植间盘的蛋白多糖和水含量降低，而胶原含量增加。生物力学动态变化表明术后早期移植间盘有失稳趋势，晚期则稳定性恢复。上述结果显示同种异体移植间盘可存活，生化代谢虽有变化但有一定的自限性，形态结构无明显改变，生物力学满足功能需要。
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| 关 键 词： | 猴子 同种异体椎间盘移植 ~3H－proline 蛋白多糖 胶原 水 生物力学 |
IntervertebralDiscAlografting |
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| RuanDike,ShenGenbiao,ZhouYong,et al..IntervertebralDiscAlografting[J].Chinese Journal of Orthopaedics,1996,16(8):511-515. |
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| Authors: | RuanDike ShenGenbiao ZhouYong |
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| Institution: | RuanDike,ShenGenbiao,ZhouYong,etal.DepartmentofOr-thopaedicSurgery,TangduHospital,4thMilitaryMedicalUniversity,Xi'an710038 |
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| Abstract: | Thisstudyistoexplore:(1)Thesurvivaloffreshdiscalograft.(2)Thechangeofra-diograph,pathology,biochemistry,discviabilityandbiomechanicsinafreshdiscalograft.Twelverhesusmonkeysweredividedinto4groups.Undergeneralanaesthesia,osteotomiesweremadeattheendplatesabout2.0mmawayfromtheL4-5intervertebraldisc,then,thefreshdiscsweretransposedintwomatchedmonkeys.The4groupsofanimalsweresacrificedafter3,6,9and12monthrespectively.Ra-diographsweretakenserialyunderanaesthesia,OnecompletelumbarspinespecimenincludingL1tosacrumfromeachgroupwereexaminedbiomechanicalyonaspinalmotiontestapparatususingamo-tionanalysissystem(Elite).Theremainingtwodiscspecimensfromeachgroupweretestedforbio-chemistry.Celviabilitywasassessedbymeasuring3H-prolineincorporation.Thediscsaboveandbelowtheoperatedlevelwereusedascontrols.Alanimalssurvivedthesurgicalprocedure.Radiographalsodemonstratedgraduallossofintervertebraldischeight,butthedischeightwasstilkept61percentofitsoriginalheightin12months.Therewasadecreaseofproteoglycanandwatercontentpostoperativ-ely.Meanwhile,therewasanincreaseinthehydroxyprolinecontentofnucleuspulpuosusoverthefol-lowupperiod,3H-prolineincorporationwasconstantlyincreasingoverthefolowupperiodatthesametime.Histologicaly,indiscspecimensfromalstages,endplatechondrocyteswereseentobeviable,therewasnoevidenceofcracksorfracturesoftheannulusfibrosus,meanwhile,therewasaregenerationofchondrocyte-likecelsinthenucleuspulposus.Noobviousimmuneresponsewasobservedinourex-periment.Biomechanicaly,hypermobilityoftheoperatedsegmentwasseenat3months,thenthehyper-mobilitywasdecreasednearlytonormallevelat12months.Thepresentresultsshowedtheinterverte-braldiscgraftssurvivedalologoustransplantation.Thestructureofintervertebraldiscsweremain-tainedaftertransfersurgery,however,thegraftingdiscswerenotcompletelynormalinitsmetabolicfunction,butthebiomechanicalfunctionwasasnormalascontrols. |
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| Keywords: | MonkeyIntervertebraldiscalografting3H-prolineProteoglycanColagenWa-terBiomechanics |
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