| CXCR4单克隆抗体对人乳腺癌细胞MCF-7凋亡的影响 |
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| 引用本文： | 马大昌,武力,王育成,马宇光,张煦.CXCR4单克隆抗体对人乳腺癌细胞MCF-7凋亡的影响[J].现代肿瘤医学,2006,14(11):1383-1385. |
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| 作者姓名： | 马大昌 武力 王育成 马宇光 张煦 |
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| 作者单位： | 1. 兰州大学第一医院乳腺病科,甘肃,兰州,730000
2. 兰州大学医学院病理学教研室,甘肃,兰州,730000 |
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| 摘 要： | 目的:探讨CXCR4单克隆抗体对人乳腺癌细胞MCF-7凋亡的影响。方法:以CXCR4单克隆抗体作用于MCF-7细胞,通过DNA梯状电泳、Hochest33258/PI荧光染色进行凋亡定性分析,以Annexin V/PI双染法定量检测MCF-7细胞的凋亡。结果:与对照组相比,CXCR4单克隆抗体作用后MCF-7细胞出现凋亡,凋亡率明显升高。结论:CXCR4单克隆抗体明显促进MCF-7细胞的凋亡,提示CXCR4/SDF-1生物学轴对乳腺癌细胞MCF-7的凋亡也有重要影响。
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| 关 键 词： | CXCR4/SDF-1 MCF-7细胞 CXCR4单克隆抗体 凋亡 |
| 文章编号： | 1672-4992-（2006）11-1383-03 |
| 收稿时间： | 2006-02-27 |
| 修稿时间： | 2006年2月27日 |
Effect of CXCR4 monoclonal antibody on apoptosis in breast cancer cell line MCF-7 |
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| MA Da-chang,WU Li,Wang Yu-cheng,MA Yu-guang,ZHANG Xu.Effect of CXCR4 monoclonal antibody on apoptosis in breast cancer cell line MCF-7[J].Journal of Modern Oncology,2006,14(11):1383-1385. |
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| Authors: | MA Da-chang WU Li Wang Yu-cheng MA Yu-guang ZHANG Xu |
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| Abstract: | Objective:To study the effect of CXCR4 monoclonal antibodies (CXCR4 mAb) on apoptosis in human breast cancer cell line MCF-7. Methods: After the addition of CXCR4 mAb, the apoptosis in MCF-7 cell were measured with DNA ladder, Hochest 33258/PI, and Annexin V/PI analysis. Results:As compared with control group, the apoptosis occurred and the apoptotic rate significantly increased in MCF-7 cell after treatment of CXCR4 mAb. Conclusion: The results suggested that CXCR4 mAb may induce apoptosis of MCF-1 cell line and CXCR4 /SDF-1 may play an important role in regulating the apoptosis of MCF-7 cell.. |
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| Keywords: | CXCR4 SDF-1 MCF-7 cell CXCR4 mAb apoptosis |
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