口腔扁平苔藓病损中肥大细胞形态及分布特点研究

目的:研究肥大细胞亚型在口腔扁平苔藓中的特征,探讨肥大细胞与扁平苔藓发病的关系。方法:应用阿辛蓝)萨红染色法显示口腔扁平苔藓组织中肥大细胞,计算成熟型和非成熟型肥大细胞的构成比;应用LUZEX-F图像分析系统检测两型肥大细胞的面积,并观察形态特点。结果:口腔扁平苔藓病损组织中非成熟型和成熟型肥大细胞构成比与正常组织间有显著性差异(P<0101);口腔扁平苔藓的丘疹型、网纹型和糜烂型间非成熟型和成熟型肥大细胞构成比有差异(P<0105);非成熟型和成熟型肥大细胞面积有显著性差异(P<0101),其胞浆内颗粒的大小、数量及分布区域均不同。结论:肥大细胞可能参与了口腔扁平苔藓的病理损害过程。

A Study on Morphology and Distribution of Mast Cells in Oral Lichen Planus

Objective:The aim of this study was to investigate the features of mast cell subtypes and relationship between mast cells and pathogenesis of oral lichen planus.Methods: Mast cells in the tissue of oral lichen planus were stained with Alcian blue /safranine in order to demonstrate mature and immature mast cells, and the constituent ratio of these two subtypes was calculated. Afterwards, LUZEX-F image pattern analysis system was applied to demonstrate the size of these two subtypes and their morphological features.Results: The constituent ratio of mature and immature types of mast cells in the tissue of oral lichen planus was significantly different from that in the normal tissue (P<0.01), and such difference was also demonstrated among different types of this malady, including papule-, net- and erosion-type of oral lichen planus (P<0.05). There was significant difference between the sizes of mature and immature mast cells (P<0.01) and also the sizes, amount and distribution of the plasma granules within these two mast cells.Conclusion: It could be implied that mast cells might play a role in the pathogenesis of oral lichen planus.