潮汕地区3种中草药中10种有机磷农药残留分布特征及风险评估

目的:建立潮汕地区3种中草药中10种有机磷农药的选择性多残留检测方法。方法:采用Qu ECh ERS法处理潮汕地区常用中草药白花蛇舌草、茅根和臭花,以气相色谱-串联质谱法为分析检测手段,建立10种有机磷农药的选择性多残留检测方法,分析不同中草药中有机磷农药总体残留水平、分布特征,并对其有机磷农药残留进行风险评估。结果:10种有机磷农药质量浓度在0.01~1.0μg/ml范围内,有机磷农药质量浓度与其峰面积之间呈良好线性关系,相关系数(r2)为0.9993~0.9997;检出限为1.0~3.0μg/kg,定量限为3.3~10.0μg/kg;加标回收率为90.3%~98.8%,相对标准偏差为1.2%~3.4%;潮汕地区随机采样的白花蛇舌草、茅根和臭花中,10种有机磷农药均未检出残留,不会对普通人群造成不可接受的长期膳食摄入风险。结论:该法准确度高、精密度好,适用于3种中草药中10种有机磷农药残留分布特征及风险评估。

Distribution Characteristics and Risk Assessment of 10 Organophosphorus Pesticide Residues in 3 Kinds of Chinese Herbal Medicines in Chaoshan

OBJECTIVE:To establish the selective multi-residue detection method for 10 organophosphorus pesticides in 3 kinds of Chinese herbal medicines in Chaoshan.METHODS:Qu EChERS method was used to treat the commonly used Chinese herbal medicines such as Hedyotis diffusa,Perotis and stinky flower in Chaoshan.Gas chromatography-tandem mass spectrometry was used as the analytical method,the selective multi-residue detection method for 10 organophosphorus pesticides was established to analyze the overall residue level and distribution characteristics of organophosphorus pesticides in different Chinese herbal medicines.The risk assessment of organophosphorus pesticide residues was performed.RESULTS:For 10 organophosphorus pesticides in the concentration range from 0.01μg/ml to 1.0μg/ml,there was good linear relationship between the mass concentration of organophosphorus pesticide and the peak area.The correlation coefficient(r2)was from 0.9993 to 0.9997,the detection limit was from 1.0μg/kg to 3.0μg/kg,the limit of quantification was from 3.3μg/kg to 10.0μg/kg.The standard recovery rate was from 90.3%to 98.8%,and the relative standard deviation was from 1.2%to 3.4%.None of the 10 organophosphorus pesticides were detected in the randomly sampled samples of Hedyotis diffusa,Perotis and stinky flowers in Chaoshan,which would not pose an unacceptable long-term dietary intake risk to the general population.CONCLUSIONS:This method has high accuracy and precision,and is suitable for the distribution characteristics and risk assessment of 10 organophosphorus pesticide residues in 3 kinds of Chinese herbal medicines.