隐球菌性脑膜炎23例脑脊液细胞学分析

目的分析隐球菌性脑膜炎脑脊液细胞学特点,比较脑脊液细胞学染色、墨汁染色、乳胶凝集实验检出隐球菌的敏感度及特异度,以提高对隐球菌性脑膜炎的认识。方法总结2008年1月至2013年2月确诊的23例隐球菌脑膜炎患者与同期住院的23例非隐球菌脑膜炎患者临床资料及脑脊液细胞学资料并予以分析。结果脑脊液细胞瑞氏吉姆萨染色检测灵敏度91.3%,明显高于墨汁染色灵敏度43.4%,差异有统计学意义(P0.05);脑脊液细胞学瑞氏吉姆萨染色检测特异度100%,明显高于乳胶凝集实验法特异度56.5%,差异有统计学意义(P0.05)。结论脑脊液细胞学检查比传统的墨汁染色法及乳胶凝集实验有更高的灵敏度及特异度,且更直接,是一种先进快捷便利的检测方法。

Cerebrospinal Fluid Cytology Analysis of 23 Cryptococcal Meningitis Cases

Aim To analyze cryptococcal meningitis cerebrospinal fluid cytology characteristics, compared with cerebrospinal fluid （CSF） cytology, ink staining, cryptococcus latex agglutination test sensitivity and specificity, and improve recognization of cryptococcus meningitis. Methods During from January 2008 to February 2013, the clinical records and cerebrospinal fluid cytological data of 23 patients with cryptococcus meningitis diagnosed in our hospital and that of 23 patients without cryptococcus meningitis in our hospital were analyzed. Results Sensitivity of cerebrospinal fluid cytology detection was 91.3%, which was significantly higher than that of ink staining detection （43.4%）, there was a significant statistical difference （x^2=12.0, P〈0.05）; Specificity of cerebrospinal fluid cytology detection was 100%, which was significantly higher than that of latex agglutination test method （56.5%）, there was a significant statistical difference （x^2=12.79, P〈0.05）. Conclusion The detection rate of cerebrospinal fluid cytology examination is more sensitive and specific than ink staining and latex agglutination test, which was an quick and convenient detection method.