骨髓间充质干细胞影响CCl_4肝纤维化大鼠的实验研究

目的探讨骨髓间充质干细胞（BMSCs）对实验性肝纤维化大鼠的保护作用。方法从健康SD雄性大鼠股骨和胫骨骨髓中获取BMSCs。选择健康雌性SD大鼠随机分为4组,每组10只。即：1对照组：1周2次,连续6周注射蓖麻油（0.2 ml/100g）;2CCl4组：1周2次,连续6周皮下注射CCl40.2 ml/100g;3CCl4/BMSCs组：大鼠注射CCl46周后,每只大鼠尾静脉注射3×106BMSCs;4CCl4/生理盐水组：大鼠注射CCl46周后,每只大鼠尾静脉注射等体积的生理盐水。BMSCs注射4周后,化学方法检测羟脯氨酸评价肝组织纤维化程度,检测血清ALT和白蛋白水平评估肝脏功能。结果与CCl4组大鼠相比,CCl4/BMSCs组大鼠血清白蛋白含量显著升高（P〈0.05）,而肝脏氨基转移酶（ALT）并未有显著降低（P〉0.05）。组织学观察,CCl4/BMSCs组肝纤维程度显著降低。提示骨髓间充质干细胞具有明显的抗纤维化能力。结论 BMSCs分化为肝细胞后,可明显的降低肝脏纤维化过程,降低胶原纤维沉积,改善肝脏组织损伤。

Effects of Bone Mesenchymal Stem Cells on CCl_4 Liver Fibrosis in Rats

Objective To study the effect of bone mesenchymal stem cells （BMSCs） on experimental liver fibrosis in rats. Methods BMSCs were derived from bone marrow obtained from femoral and tibial bones of male SD rats. Rats were divided into four groups ： control, CC14, CC14 plus BMSCs, and BMSCs. After injection of BMSCs for 4 weeks, liver tissue was observed histopathologically and liver functions were estimated by ALT and serum albumin for all groups. Hydroxyproline content in liver tissue was assessed by chemical methods to detect liver fibrosis. Results The content of serum albumin increased significantly in the CCI4/BMSCs group compared to the CC14 group （ P 〈 0.05 ）. Liver enzyme ALT had a decreasing tend but no significant change in the CC14/ BMSCs group compared to the CC14 group （ P 〉 0.05 ）. The degree of liver fiber decreased significantly by his- topathological observation in the CC14/BMSCs group. Conclusion BMSCs slowed down the liver fibrosis, re- duced collagen deposition, and improved the structure of liver tissue injury by differentiating into hepatocytes.