眼组织的透射电镜制样方法

介绍一种眼组织透射电子显微镜标本制备方法。组织先用４％多聚甲醛－２．５％戊二醛混合固定液预固定，１％四氧化锇固定液后固定，丙酮逐级脱水，延长Ｅｐｏｎ８１２包埋液的浸透时间并包埋，用玻璃刀或钻石刀作超薄切片，醋酸主枸橼酸铅双重染色，Ｈ６００－Ⅳ型透射电子显微镜观察。此方法的优点在于眼组织各层超微结构保存良好，没有损伤及变性，我们对该方法的操作要点作了讨论。

A Method of Preparing the Section of Eye Tissue for Transmission Electron Microscopy

A method of preparing the section of eye tissue for transmission electron microscopy (TEM) is recommended. The tissue was prefixed with a mixed solution of 4% paraformaldehyde and 2.5% glutaraldehyde and followed by softening in 3% EDTA solution for 20 minutes, then the tissue was fixed in 1% osmium tetroxide, dehydrated in series acetone, infiltrated in Epox 812 for a longer, and embeded. Ultrathin sections were cut with glass knives or diamond knife, stained with uranyl acetate and lead citrate and examined with H600-IV. The advantage of this method is that the ultrastructures of tissues are well preserved without any damage and distortion. The main points of the procedure of preparation have been discussed.