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| RP-HPLC法同时测定虎杖提取物中4种组分含量 | |
| 引用本文： | 欧彩娟,柳文媛,吴玉兰,杨治华.RP-HPLC法同时测定虎杖提取物中4种组分含量[J].广东药学院学报,2011,27(6):609-612. |
| 作者姓名： | 欧彩娟 柳文媛 吴玉兰 杨治华 |
| 作者单位： | 1. 深圳市龙岗中心医院,广东 深圳,518000 2. 中国药科大学深圳研究院,广东 深圳,518057 3. 浙江花园生物股份有限公司,浙江 杭州,310000 |
| 摘 要： | 目的 建立RP-HPLC法测定虎杖提取物中白藜芦醇苷、白藜芦醇、大黄素和大黄素甲醚的含量.方法 采用Kromasil C8色谱柱,以甲醇-水为流动相进行梯度洗脱.流速为1 mL/min;检测波长为290nm；进样量为20μL；柱温为30℃.结果 虎杖提取物中白藜芦醇苷、白藜芦醇、大黄素和大黄素甲醚分别在10.54... |
| 关 键 词： | 高效液相色谱法 虎杖 白藜芦醇苷 白藜芦醇 大黄素 大黄素甲醚 |
RP-HPLC method for simultaneous determination of four components in extract from Polygonum cuspidatum |
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| OU Cai-juan,LIU Wen-yuan,WU Yu-lan,YANG Zhi-hua.RP-HPLC method for simultaneous determination of four components in extract from Polygonum cuspidatum[J].Academic Journal of Guangdong College of Pharmacy,2011,27(6):609-612. | |
| Authors: | OU Cai-juan LIU Wen-yuan WU Yu-lan YANG Zhi-hua |
| Institution: | 1.Longgang District Central Hospital of Shenzhen,Shenzhen 518000,China;2.Research Institute of China Pharmaceutical University In Shenzhen,Shenzhen 518057,China;3.Zhejiang Garden Biochemical High-Tech Co.,Ltd,Hangzhou 310000,China) |
| Abstract: | Objective To develop a simple and rapid RP-HPLC method for simultaneously determination of polydatin,resveratrol,emodin and physcion in extract from Polygonum cuspidatum.Methods The HPLC method was performed on a Kromasil C8 at 30 ℃,and the mobile phase was methanol-water with gradient elution at a flow rate of 1 mL/min.The detection wave length was 290 nm,and 20 μL was injected.Results The linear range of polydatin,resveratrol,emodin and physcion were 10.54-52.7 μg/mL,4.5-22.5 μg/mL,4.92-49.2μg/mL and 3.51-35.1 μg/mL,respectively.The average recovery ratio were 97.70%,97.20%,102.6% and 100.0%,with the relative standard deviations（RSD）of 2.36%,1.89%,1.23% and 3.67%,respectively.Conclusion The method proved to be rapid,accurate and simple,suitable for the quality control of extract of Polygonum cuspidatum. |
| Keywords: | RP-HPLC Polygonum cuspidatum polydatin resveratrol emodin physcion |
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