桑叶有效部位对胰岛素抵抗HepG2细胞体外糖代谢的影响

目的 探讨桑叶水提物(mulberry leaves extracts,MLE)、桑叶多糖(mulberry leaves polysaccharides,MLP)、桑叶黄酮(mulberry leaves flavonoids,MLF)对胰岛素抵抗HepG2(IR-HepG2)细胞糖代谢的作用.方法 采用高浓度胰...

Effect of bioactive components in mulberry leaves on glucose metabolism in insulinresistant HepG2 cells

Objective To explore the effect of bioactive components in mulberry leaves,including mulberry leaves extracts（MLE）,mulberry leaves polysaccharides（MLP） and mulberry leaves flavonoids（MLF）,on glucose metabolism in insulin-resistant HepG2 cells.Methods The insulin resistant models of HepG2 cells were induced by high concentration of insulin for 24 h.The glucose consumption of cells,the liver glycogen,hexokinase（HK） and pyruvate kinase（PK） were measured after incubation with bioactive components in mulberry leaves.Results MLE and MLF advanced glucose uptake in insulin-resistant HepG2 cells.The liver glycogen synthesis,HK secretion and PK secretion increased significantly after incubation with MLE.Conclusion MLE and MLF can improve the glucose consumptions in insulin-resistant model of HepG2 cells.MLE can enhance HK and PK vitality,promote blood glucose to enter the liver cells and increase glycogen synthesis.MLE may regulate the glucose metabolism and improve the symptoms in diabetes mellitus.