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| HPLC法测定乌参醒脑滴丸中二苯乙烯苷的含量 | |
| 引用本文： | 范辉,顾泉琳,何梓蔚,黄睿,贝伟剑. HPLC法测定乌参醒脑滴丸中二苯乙烯苷的含量[J]. 广东药学院学报, 2011, 27(6): 601-603. DOI: 10.3969/j.issn.1006-8783.2011.06.012 |
| 作者姓名： | 范辉 顾泉琳 何梓蔚 黄睿 贝伟剑 |
| 作者单位： | 1. 广东药学院中医药研究院,广东 广州 510006;广东药学院药科学院,广东 广州 510006 2. 广东药学院中医药研究院,广东 广州,510006 3. 广东药学院药科学院,广东 广州,510006 |
| 基金项目： | 广东药学院2007年度人才引进基金资助项目，广州市科技计划助资项目 |
| 摘 要： | 目的建立高效液相色谱法测定乌参醒脑滴丸中2,3,5,4＇-四羟基二苯乙烯-2-O-β-D-葡萄糖苷（二苯乙烯苷）含量的方法。方法采用SunFireTM C18（250 mm×4.6 mm,5.0μm）色谱柱,柱温为30℃;以乙腈-水（体积比20∶80）为流动相,流速为1.0 mL.min-1;检测波长为320 nm。结果二苯乙烯苷在12.75～85.0μg.mL-1范围内与峰面积呈良好的线性关系,平均回收率为99.8%,RSD为2.16%。结论该法可用于乌参醒脑滴丸中二苯乙烯苷的含量测定。 |
| 关 键 词： | 乌参醒脑滴丸 二苯乙烯苷 高效液相色谱法 含量测定 |
Determination of stilbene glucoside in Wushen Xingnao drop pill by HPLC |
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| FAN Hui,GU Quan-lin,HE Zi-wei,HUANG Rui,BEI Wei-jian. Determination of stilbene glucoside in Wushen Xingnao drop pill by HPLC[J]. Academic Journal of Guangdong College of Pharmacy, 2011, 27(6): 601-603. DOI: 10.3969/j.issn.1006-8783.2011.06.012 | |
| Authors: | FAN Hui GU Quan-lin HE Zi-wei HUANG Rui BEI Wei-jian |
| Affiliation: | 1(1.Institute of Chinese Medicinal Sciences;2.School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China) |
| Abstract: | Objective To establish a method for the determination of stilbene glucoside in Wushen Xingnao（WSXN） drop pill with HPLC.Methods Chromatography was performed on a SunFireTMC18（250 mm ×4.6 mm,5.0 μm） at 30 ℃.The mobile phase was composed of acetonitrile-water（20∶80）with a flow rate of 1.0 mL·min-1.The detection wavelength was 320 nm.Results The calibration curve of stilbene glucoside was linear in the range of 12.75-85.0 μg·mL-1.The average recovery for stilbene glucoside was 99.8%（RSD=2.16%）.Conclusion The method can be used for quantitative determination of Wushen Xingnao drop pill. |
| Keywords: | Wushen Xingnao drop pill stilbene glucoside HPLC assay |
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