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| HPLC法测定西藏主产区冬虫夏草中尿苷和腺苷的含量 | |
| 引用本文： | 周伟平,高晓霞,代安国,熊卫萍,谢荣,钟兆健,章卫民. HPLC法测定西藏主产区冬虫夏草中尿苷和腺苷的含量[J]. 广东药学院学报, 2011, 27(6): 591-595. DOI: 10.3969/j.issn.1006-8783.2011.06.010 |
| 作者姓名： | 周伟平 高晓霞 代安国 熊卫萍 谢荣 钟兆健 章卫民 |
| 作者单位： | 1. 广东药学院 药科学院,广东 广州,510006 2. 西藏农牧科学院蔬菜研究所,西藏 拉萨,850000 3. 广东省微生物研究所,广东 广州,510070 |
| 基金项目： | 国家科技支撑计划项目，国家自然科学基金项目，广东省科学院分析测试基金项目 |
| 摘 要： | 目的 建立HPLC法同时测定西藏产冬虫夏草中尿苷及腺苷的含量.方法 采用Agilent Eclipse XDB-C18(250 mm ×4.6 mm,5 μm)色谱柱,检测波长260 nm,以甲醇-水为流动相梯度洗脱,流速1.0mL/min,柱温33℃.结果 尿苷和腺苷分别在32.95～439.3 μg/mL(r =... |
| 关 键 词： | 冬虫夏草 尿苷 腺苷 HPLC法 含量测定 |
Analysis of uridine and adenosine in Ophiocordyceps sinensis from the main producing areas of Tibet by HPLC |
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| ZHOU Wei-ping,GAO Xiao-xia,DAI An-guo,XIONG Wei-ping,XIE Rong,ZHONG Zhao-jian,ZHANG Wei-ming. Analysis of uridine and adenosine in Ophiocordyceps sinensis from the main producing areas of Tibet by HPLC[J]. Academic Journal of Guangdong College of Pharmacy, 2011, 27(6): 591-595. DOI: 10.3969/j.issn.1006-8783.2011.06.010 | |
| Authors: | ZHOU Wei-ping GAO Xiao-xia DAI An-guo XIONG Wei-ping XIE Rong ZHONG Zhao-jian ZHANG Wei-ming |
| Affiliation: | 1.School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;2.Institute of Vegetable Sciences,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lasa 850000,China; 3.Guangdong Institute of Microbiology,Guangzhou 510070,China) |
| Abstract: | Objective To establish a HPLC method to determine uridine and adenosine in Ophiocordyceps sinensis from the main producing areas of Tibet. Methods Uridine and adenosine were separated on Agilent C18 column （250 mm × 4.6 mm,5 μm） and detected at 260 nm. The mobile phase was consisted of methanol and water using a gradient elution. The column temperature was 33 ℃ and the flow rate was 1.0 mL/min. Results The linearity of uridine and adenosine were 32.95 - 439.3 μg/mL （ r = 0. 999 9 ） and 3. 486 -46.48 μg/mL（r =0. 999 9） ,and the average recovery ratios were 98.5% and 95.6%. The RSD were 1.8% and 2.4% ,respectively. The contents of uridine and adenosine ranged from 1. 209 to 2. 192 mg/g and 0.083 8 to 0. 884 mg/g respectively. Conclusion The method is simple and accurate, and it can be used for the determination of uridine and adenosine quickly. It provided a reference to control the quality of Ophiocordyceps sinensis from Tibet. |
| Keywords: | Ophiocordyceps sinensis uridine adenosine HPLC determination |
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