胸苷酸合成酶基因表达与结肠直肠癌复发的关系及其临床意义

目的 探讨胸苷酸合成酶（ＴＳ）基因表达与结、直肠癌复发的关系及其对临床治疗的影响。方法 对６８例结、直肠癌患者采用ＲＴ－ＰＣＲ方法检测大肠癌原发灶、癌旁肠粘膜、局部复发灶、腹腔盆腔内播散和肝转移灶中ＴＳ基因的表达;用Ｗｅｓｔｅｒｎ Ｂｌｏｔ方法检测ＴＳ蛋白达表。结果 ６８例结肠直肠癌原发灶、局部复发灶、腹腔及盆腔内播散和肝转移灶中ＴＳ基因表达阳性率分别为２２．１％（１５／６８）、８８．５％（２３／

Expression of thymidylate synthase gene and recurrence of colorectal carcinoma: their relation and clinical significance

ObjectiveTo study the relationship between expression of thymidylate synthase (TS) gene and recurrence of colorectal carcinoma and its effect on clinical treatment. Methods RT PCR was used to detect the expression of TS gene in primary foci, paratumoral intestine mucosa, local recurrence, abdominal pelvic dissemination and hepatic metastasis in 68 cases of colorectal carcinoma, and TS protein was examined with western blot. Results In 68 cases of colorectal carcinoma, the expression of TS gene in primary foci was 22 1%(15/68); and the positive rates of TS gene expression in local recurrence, abdominal pelvic dissemination and hepatic metastasis were 88 5%(23/26), 85 0%(17/20), 40 9%(9/20) respectively. The rates of TS protein expression in primary foci, local recurrence, abdominal pelvic dissemination and hepatic metastasis were 22 1%(15/68), 84 6%(22/26), 80 0%(16/20), 36 4%(8/22) respectively. The negative expression of TS gene and TS protein was detected in paratumoral intestinal mucosa. The results of TS gene and TS protein expression were identical with those the two methods. The positive rates of TS gene and TS protein expression in diversified recurrence foci and metastasis were higher than those in primary foci ( P <0 01). The differences of TS gene and TS protein expression rates between recurrence and hepatic metastasis were significant ( P <0 01). The expression rates of TS gene and TS protein in local recurrence and abdominal pelvic dissemination tissues were higher than those in hepa tic metastasis. Conclusion Overexpression of TS gene plays an important role in the process of local recurrence and abdominal pelvic dissemination of colorectal carcinoma.