| 乙醇诱导肝癌细胞凋亡的实验研究 |
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| 引用本文： | 荆绪斌,李涛,胡辉,曾庆馀. 乙醇诱导肝癌细胞凋亡的实验研究[J]. 中国基层医药, 2004, 11(8): 905-906 |
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| 作者姓名： | 荆绪斌 李涛 胡辉 曾庆馀 |
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| 作者单位： | 515041,汕头大学医学院第一附属医院消化内科 |
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| 摘 要： | 目的研究低浓度乙醇对肝癌细胞的影响及作用机制。方法采用噻唑蓝比色法观察乙醇对细胞增殖的影响；采用流式细胞仪法观察乙醇对肝癌细胞内活性氧的影响；采用琼脂凝胶电泳法观察DNA片断化条带。结果低浓度乙醇可明显抑制细胞增殖；乙醇作用于细胞后2h内引起活性氧产生，24h后细胞出现典型的凋亡表现。结论乙醇能够通过诱导细胞内活性氧的产生引起细胞凋亡，呈明显的剂量依赖性。
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| 关 键 词： | 乙醇 肝癌 细胞凋亡 实验 癌细胞 作用机制 |
| 修稿时间： | 2004-04-13 |
Study of apoptosis of hepatic cell carcinoma cell line induced by ethanol |
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| JING Xubin,LI Tao,HU Hui,et al.. Study of apoptosis of hepatic cell carcinoma cell line induced by ethanol[J]. Chinese Journal of Primary Medicine and Pharmacy, 2004, 11(8): 905-906 |
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| Authors: | JING Xubin LI Tao HU Hui et al. |
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| Affiliation: | JING Xubin,LI Tao,HU Hui,et al.Department of Gastroenterology,The First Affiliated Hospital,Shantou University,Medical College,Shantou,Guangdong,515041,China |
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| Abstract: | Objective To study the mechanism of low concentration ethanol on proliferation of the hepatic cell carcinoma cell line.Methods MTT colerimetry was used to evaluate the influence of ethanol on proliferation of cells.Flow cytometry was used to test reactive oxygen species(ROS) levels in cells.Electrophroresis in 1.5% agarose gels was used to assess the DNA laddering.Results Low concentration ethanol could suppress proliferation of tumor cell line.ROS generation increased obviously after the cell line incubated in ethanol 2 hours.Typical DNA ladder was observed after the cell line incubated in ethanol 24 hours.Conclusion Low concentration ethanol can induce the apoptosis of hepatic cell carcinoma cell line by generation of ROS in a dose-dependent manner. |
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| Keywords: | Ethanol Hepatic carcinoma Cell apoptosis |
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