化疗药物与细胞因子协同作用引起小鼠T淋巴瘤细胞凋亡

本实验旨在了解化疗药物和细胞因子协同作用对细胞凋亡的影响。在培养的小鼠Ｔ淋巴瘤ＲＭＡ细胞系中加化疗药物地塞米松（ＤＥＸ）、足叶乙甙（ＶＰ１６）、三氧化二砷（Ａｓ２Ｏ３）及维甲酸（ＡＴＲＡ）以及培养细胞中先分别与细胞因子ＩＬ－２，ＩＬ－６和ＧＭ－ＣＳＦ共同培养后再加入上述药物，观察对引起细胞凋亡的影响。四种化疗药均能抑制ＲＭＡ细胞增殖，ＤＥＸ，ＶＰ１６和Ａｓ２Ｏ３可以诱导细胞凋亡，单独使用ＡＴＲＡ不

Synergistic Induction of Apoptosis by Chemotherapeutic Drugs and Cytokines in Mouse T-Lymphoma Cell Line

The objective of the study is to find out the synergistic effect on apoptosis resulting from the combination of chemotherapeutic drugs and some cytokines. Dexamethasone(DEX), etoposide(VP16), arsenic trioxide(As 2O 3 ) and alltrans-retinoic acid (ATRA) were added to the murine T lymphoma cell line RMA as well as to the cells preincubated with IL-2, IL-6 or GM-CSF, respectively. The effect on apoptosis was observed. All four chemotherapeutic drugs inhibited the cell proliferation. DEX,VP16 or As 2O 3,except ATRA, singly induced apoptosis of RMA cells. The DEX concentration of inducing apoptosis was reduced when it was added together with ATRA. IL-2,IL-6 and GM-CSF did not induce apoptosis when the cytokines were added to RMA separately, however, apoptosis could be induced by combination of IL-2 and IL-6. The cytokines facilitated the apoptotic action of chemotherapeutic drugs, the drug concentration for inducing apoptosis decreased and the time period of starting apoptosis shortened. The results demonstrated that there was synergistic effect of chemotherapeutic drugs and some cytokines for induction of apoptosis. It raised an experimental basis for combination of chemotherapeutic drugs and some cytokines, especially IL-2, in the treatment of malignant lymphoma.