奥曲肽对人肝癌诱导肿瘤血管生成的抑制作用

目的　探讨生长抑素类似物奥曲肽对人肝癌诱导肿瘤血管生成的抑制作用。方法　采用人肝癌LCI D2 0裸鼠角膜微囊移植模型 ,利用体视显微镜和显微数码摄像系统 ,动态观测奥曲肽对人肝癌诱导肿瘤血管生成能力的影响。应用人肝癌LCI D2 0皮下移植瘤模型 ,采用CD34免疫组织化学SP法检测肿瘤标本微血管密度 (MVD) ,观察奥曲肽对人肝癌诱导的肿瘤血管生成和皮下移植瘤生长的影响。结果　LCI D2 0肝癌组织移植裸鼠角膜微囊能够诱导角膜新生血管形成 ;与对照组相比 ,奥曲肽组动物角膜新生血管芽生延迟 ,新生毛细血管网稀疏、生长缓慢 ;术后第 7、9、12、15、18、2 1天 ,奥曲肽组人肝癌诱导的角膜新生血管积分比对照组减少 (P <0 0 5 )。奥曲肽对LCI D2 0皮下肿瘤生长具有抑制作用 ,免疫组化研究表明 ,治疗组肿瘤内MVD(2 1 7± 4 2 7)比对照组MVD(31 8± 3 87)减少 (P <0 0 1)。结论　生长抑素类似物奥曲肽对人肝癌诱导肿瘤血管生成具有抑制作用 ,可能为肝癌的治疗提供一个新的途径。

Effect of octreotide on angiogenesis induced by hepatocellular carcinoma in vivo

Objective To investigate the effect of somatostatin analogue octreotide on angiogenesis induced by hepatocellular carcinoma (HCC) in vivo. Methods Using LCI-D20 corneal micropocket model in nude mice, angiogenesis was dynamically observed under a stereoscopic zoom microscope and a digital camera system ,and the effect of octreotide on angiogenesis was evaluated .Results When the animals received sys temic octreotide treatment,the angiogenesis response in mice cornea was moderate ,the appearance of vascular buds was delayed and the new capillaries were sparse and grew slowly .Compared with the control group ,on day 7,9,12 ,15 ,18 and 21 after implantation ,the neovascularization induced by HCC in mice cornea was marked ly inhibited in octreotide-treated group (P <0 0 5 ) .Conclusion The somatostatin analogue octreotide is able to inhibit angiogenesis induced by HCC in vivo and may provide a new approach to the treatment of HCC .