Assignment of the human gene for δ aminolevulinate dehydrase to chromosome 9 by somatic cell hybridization and specific enzyme immunoassay |
| |
| Authors: | C. BEAUMONT,C. FOUBERT&dagger ,B. GRANDCHAMP,D. WEIL&dagger ,VAN CONG N'GUYEN&dagger ,M. S. GROSS&dagger ,Y. NORDMANN |
| |
| Affiliation: | *Department of Biochemistry, Faculty of Medicine Xavier Bichat, Hôpital Louis Mourier, 92701 Colombes, France;†Unitéde Recherche de Génétique Médicale, INSERM U12, Hôpital des Enfants Malades. 149 rue de Sèvres, 75730 Paris cedex, France |
| |
| Abstract: | A non-competitive enzyme immunoassay specific for δ aminolevulinate dehydrase has been devised and applied to rodent–human hybrid cell lines. Two different conditions have been used, one specific for the human enzyme and the other indicative of both rodent and human enzymes. The ratio of the values obtained under the two conditions was used to discriminate between positive and negative clones. By this method the gene for ALA dehydrase has been assigned to chromosome 9. |
| |
| Keywords: | |
|
|
