调心方药物血清对动物阿尔茨海默病相关的tau蛋白磷酸化的调节作用

目的：研究调心方对动物阿尔茨海默病相关的tau蛋白磷酸化的调节及其可能的作用机制。方法：采用冈田酸（Okadaic acid）处理NG108细胞模型，MTT比色法、蛋白印迹法、免疫共沉淀等方法进行测定。结果：MTT方法观察到磷酸脂酶抑制剂冈田酸（20nmol/L）处理NG108细胞12h能导致细胞活性明显下降，调心方药物血清能改善细胞的活性；多种tau蛋白抗体免疫印迹法见调心方药物血清能逆转冈田酸导致的NG108细胞tau蛋白过度磷酸化。免疫共沉淀研究观察到调心方提取液抑制tau蛋白与可调节tau蛋白磷酸化的早老蛋白－1（Presenilin-1）的结合。结论：调心方能抑制tau蛋白的过度磷酸化，其部分机制可能与调心方调节早老蛋白－1与tau蛋白结合有关。

Regulatory Effect of Tiaoxin Recipe Drug Serum on Animal''''s Alzheimer Disease Related tau Protein Phosphorylation

Objective: To study the regulatory effect and possible mechanism of Tiaoxin Recipe (TXR) on animal's Alzheimer disease related tau protein phosphorylation. Methods: NG108 cell model was treated with Okadaic acid and related parameters were determined using MTT staining, immunoblot, coimmunoprecipitation assay, etc. Results: Shown by MTT staining, NG108 cell activity decreased significantly after treated with Okadaic acid for 12 hrs, which could be ameliorated by TXR rat serum. Revealed by immunoblot mehtod, the Okadaic acid induced elevation of phosphorylated tau protein could partly be reversed after co treated with TXR rat serum. TXR extract could inhibit the binding of tau protein with presenilin 1, which may regulate the tau protein phosphorylation, and could be observed by coimmunoprocipitation. Conclusion: TXR could inhibit tau protein hyperphosphorylation, which might partially be due to the TXR caused binding of presenilin 1 with tau protein.