牙龈卟啉单胞菌临床菌株的毒力分析

目的:利用啮齿动物模型,比较分析中国人群口腔中牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)临床菌株的毒力。方法:采用小鼠脓肿形成实验,将6株从牙周炎患者牙周袋中分离获得的P.gingivalis临床菌株L2、L3、L4、L5、L11和L12分别注射至小鼠后背中央,观察其临床症状,并与国际标准菌株ATCC33277和W83进行比较,分析临床菌株的毒力特性。采用SPSS11.5软件包对数据进行统计学分析。结果:L3、ATCC33277和W83诱发小鼠产生原发性脓肿和轻微的全身反应,L2、L5、L11、L12则诱发小鼠产生远处转移性病变和严重的全身反应。结论:中国牙周炎患者口腔中的P.gingivalis临床菌株与国际标准菌株相比,在诱发实验鼠发生局部脓肿方面,具有一定程度的相似性,但临床菌株间的毒力特征具有较大差异。

Virulent properties of Porphyromonas gingivalis clinical strains

PURPOSE:To investigate the virulent properties of P.gingivalis clinical strains.METHODS:By using mouse abscess model(MAM),six P.gingivalis clinical strains,L2,L3,L4,L5,L11 and L12 were subcutaneously inoculated into the central back of BALB/C mice.The clinical signs of the mice were observed and the virulent properties of the clinical strains were analyzed by comparison with type strains of W83 and ATCC 33277.SPSS11.5 software package was used for statistical analysis.RESULTS:According to the criteria established in previous reports,L3,ATCC33277,and W83 produced a localized abscess at the site of injection and were categorized as noninvasive.L2,L5,L11,and L12-induced lesions spread to distant organs and may produce severe systemic reactions and these strains were classified as invasive.CONCLUSIONS: Clinical strains from Chinese patients showed similarities to type strains in MAM.The virulent properties of P.gingivalis clinical strains are quite different from each other.