原发及转移性卵巢癌组织中绒毛蛋白、细胞角蛋白7及20的表达

目的:检测原发及转移性卵巢癌组织中绒毛蛋白(Villin)、细胞角蛋白7及20(CK7、CK20)的表达.方法:采用免疫组织化学方法检测卵巢原发性腺癌45例(浆液性腺癌18例、黏液性腺癌15例、内膜样腺癌12例)、结肠转移性卵巢癌15例、原发结肠腺癌20例、正常卵巢组织10例中Villin、CK7和CK20的表达. 结果:正常卵巢、原发性卵巢腺癌、结肠转移性卵巢癌及原发性结肠腺癌组织间比较,Villin、CK7和CK20的阳性表达率差异均有统计学意义(P＜0.001);且原发卵巢癌组织中Villin的阳性表达率低于CK20(P＜0.005).结论:Villin、CK7和CK20的测定对鉴别原发及转移性卵巢癌有重要意义,Villin在特异性方面强于CK20 .

Expression of Villin, CK7, and CK20 in primary and metastatic ovarian carcinoma tissue

Aim: To investigate the utility of a limited panel consisting of Villin, CK7, and CK20 differentiating primary and metastatic ovarian tumor. Methods: Immunohistochemical detection of Villin, CK7, and CK20 were performed on 45 cases of primary ovarian adenocarcinoma (18 cases of serous,15 cases of mucinous, and 12 cases of endometrioid),15 cases of colonic carcinoma metastatic to the ovaries, 20 cases of primary colonic carcinoma, and 10 cases of normal ovary tissue. Results: The positive expression rates of Villin, CK7, and CK20 in normal ovary tissue, primary of ovarian adenocarcinoma tissue, colonic carcinoma metastatic to the ovaries, and primary colonic carcinoma tissue were significantly different (P<0.001). And the positive expression rate of CK20 was higher than that of Villin in primary ovarian tumor tissue (P<0.005).Conclusion: A limited panel of Villin, CK7, and CK20 is helpful in distinguishing primary from metastatic ovarian carcinoma. And Villin is more specific than CK20 as it is lower expression in primary ovarian carcinoma.