| Notch1基因C端特征结构域pcDNA3.1-AN真核表达载体的构建 |
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| 引用本文： | 鲁照明,王莉莉,张爱琴,许培荣,刘红涛,薛乐勋. Notch1基因C端特征结构域pcDNA3.1-AN真核表达载体的构建[J]. 郑州大学学报(医学版), 2007, 42(2): 228-230 |
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| 作者姓名： | 鲁照明 王莉莉 张爱琴 许培荣 刘红涛 薛乐勋 |
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| 作者单位： | 郑州大学细胞生物学研究室,郑州大学第一附属医院,郑州,450052;河南省中医学院第一附属医院检验科,郑州,450000 |
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| 基金项目： | 河南省医学科技攻关计划项目 |
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| 摘 要： | 目的:扩增人胎盘组织Notch1基因C端特征性结构域ANK和NLS(简称AN),并构建pcDNA3.1-AN真核表达载体.方法:按照GenBank中Notch1序列,设计-对引物,利用RT-PCR方法从人胎盘组织中获取AN cDNA片段,将其克隆至真核表达质粒pcDNA3.1( )中,构建重组真核表达载体pcDNA3.1-AN.结果:所获得的序列是Notch1特征性的AN结构域.结论:成功构建了pcDNA3.1-AN真核表达载体.
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| 关 键 词： | Notch1 ANK结构域 NLS结构域 pcDNA3.1 |
| 收稿时间： | 2006-05-11 |
| 修稿时间： | 2006-05-11 |
Construction of Notch1 AN domain eukaryotic expression vector pcDNA3.1-AN |
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| LU Zhaoming,WANG Lili,ZHANG Aiqin,XU Peirong,LIU Hongtao,XUE Lexun. Construction of Notch1 AN domain eukaryotic expression vector pcDNA3.1-AN[J]. Journal of Zhengzhou University: Med Sci, 2007, 42(2): 228-230 |
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| Authors: | LU Zhaoming WANG Lili ZHANG Aiqin XU Peirong LIU Hongtao XUE Lexun |
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| Affiliation: | 1. Laboratory for Cell Biology, Zhengzhou University, Zhengzhou 450052; 2. Department of Clinical Laboratory, the First Affiliated Hospital, Henan College of Traditional Chinese Medicine, Zhengzhou 450000 |
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| Abstract: | Aim: To amplify Notch1 ANK and NLS (AN) domain from human placenta tissue and construct eukaryotic expression vector pcDNA3.1-AN.Methods:Notch1 AN domain was obtained by RT-PCR method from placenta tissue using a pair of primers designed according GenBank database,and was inserted into eukaryotic expression vector pcDNA3.1( ). Results: The sequence obtained was AN domain of Notch1.Conclusion: The eukaryotic expression vector pcDNA3.1 ( )-AN is constructed successfully. |
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| Keywords: | Notchl ANK domain NLS domain pcDNA3.1 |
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