| 河南汉族冠心病患者N5,N10-亚甲基四氢叶酸还原酶基因C677T多态性检测 |
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| 引用本文： | 焦昆立,赵洛沙,于海东,于辉,刘传红,郑红.河南汉族冠心病患者N5,N10-亚甲基四氢叶酸还原酶基因C677T多态性检测[J].郑州大学学报(医学版),2007,42(3):426-428. |
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| 作者姓名： | 焦昆立 赵洛沙 于海东 于辉 刘传红 郑红 |
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| 作者单位： | 郑州大学第一附属医院心内科,郑州,450052;郑州大学基础医学院细胞生物学与医学遗传学教研室,郑州,450001 |
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| 基金项目： | 河南省高等学校杰出人才创新工程项目
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河南省杰出青年科学基金 |
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| 摘 要： | 目的:探讨N5,N10-亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性与冠心病(CHD)的相关性.方法:应用PCR-限制性片断长度多态性(RFLP)技术,检测208例CHD患者和500名健康人(正常组)MTHFR C677T基因多态.结果:MTHFR基因第677位存在2种等位基因(C和T)、3种基因型(C/C、C/T、T/T).CHD组C/C、C/T、T/T 3种基因型频率分别为15.4%、45.7%、38.9%,T等位基因频率为61.8%,C等位基因频率为38.2%;正常组C/C、C/T、T/T型频率分别为35.6%、44.0%、20.4%,T等位基因频率为42.4%,C等位基因频率为57.6%.CHD组T/T型及T等位基因频率均高于对照组(P＜0.05).结论:MTHFR基因C677T多态性可能与河南汉族人群CHD的发生有关.
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| 关 键 词： | 亚甲基四氢叶酸还原酶 基因多态性 同型半胱氨酸 冠心病 河南 汉族 |
| 收稿时间： | 2006-11-18 |
| 修稿时间： | 2006-11-18 |
Detection of polymorphisms of methylenetetrahydrofolate reductase C677T in patients with coronary heart disease in Henan Han population |
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| JIAO Kunli,ZHAO Luosha,YU Haidong,YU Hui,LIU Chuanhong,ZHENG Hong.Detection of polymorphisms of methylenetetrahydrofolate reductase C677T in patients with coronary heart disease in Henan Han population[J].Journal of Zhengzhou University: Med Sci,2007,42(3):426-428. |
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| Authors: | JIAO Kunli ZHAO Luosha YU Haidong YU Hui LIU Chuanhong ZHENG Hong |
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| Institution: | 1 Department of Cardiology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 ;2 Department of Cell Biology and Medical Genetics, College of Basic Medical Sciences, Zhengzhou University ,Zhengzhou 450001 |
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| Abstract: | Aim: To explore the relationship between methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism and coronary heart disease (CHD). Methods: A total of 208 patients with CHD and 500 normal controls were subjected to detect MTHFR C677T gene polymorphism using PCR-RFLP. Results: There were three kinds of genotype: C/C, C/T and T/T. In CHD group, the frequencies of C/C, C/T and T/T genotype were 15.4%, 45.7% and 38.9%, respectively. T and C allele frequencies were 61.8% and 38.2%, respectively. In the control group, the frequencies of C/C, C/T and T/T genotype were 35.6%, 44.0%, and 20.4%, respectively. T and C alleles frequencies were 42.4% and 57.6%. The frequencies of T/T genotype and T allele in CHD group were significantly higher than those in the control group (P<0.05). Conclusion: There may be an association between MTHFR C677T polymorphism and CHD in Henan Han population. |
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| Keywords: | methylenetetrahydrofolate reductase polymorphism homocysteinc acid coronary heart disease Henan province Han population |
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