全反式维甲酸对A549细胞株增殖和Caspase-3蛋白表达的影响

目的:探讨全反式维甲酸(ATRA)对A549肺腺癌细胞增殖和凋亡相关蛋白Caspase-3表达的影响.方法:①取对数生长期的人肺腺癌细胞株A549细胞,消化后于96孔板上按1×10-5 mol/L,1×10-6 mol/L,1×10-7 mol/L 3组ATRA药物浓度组和空白对照组培养,采用四甲基偶氮唑蓝(MTT)比色法观察ATRA作用2 d,4 d,6 d对A549细胞增殖的影响.②用Hoechest33258荧光染色观察1×10-5 moL/LATRA培养24 h后A549细胞胞核形态变化.③利用免疫印迹法(Western blot)检测经1×10-7 mol/L,1×10-6 mol/L,1×10-5 mol/L ATRA作用12 h,24 h后A549细胞中Caspase-3蛋白表达的变化.结果:ATRA处理组与空白对照组相比细胞的增殖活性明显受到抑制(P＜0.05);经1×10-5 mol/L ATRA处理后的细胞呈典型的凋亡核固缩表现,胞核呈致密浓染的颗粒状或块状荧光.ATRA处理组的Caspase-3蛋白表达与空白对照组相比明显增高.结论:ATRA可抑制人肺癌细胞株A549的增殖,并通过上调Caspase-3蛋白表达诱导细胞的凋亡.

Effect of ATRA on the expression of Caspase-3 in human lung adenocarcinoma cell line A549 and cell proliferation

Aim: To investigate the effect of ATRA on the proliferation of lung cancer cell and expression of Caspase-3 in lung cancer cells.Methods:The effects of ATRA on the proliferation of lung cancer cells were measured by MTT method. Hoechest33258 fluorescent dyeing was used to observe ATRA-induced morphological changes. The expression of activated Caspase-3 protein was detected by Western blot.Results: Cell proliferation was significantly inhibited by ATRA(P<0.05), the morphological changes of apoptosis such as nuclear fragmentation or condensed particles were identified after incubation with ATRA, and the expression of activated Caspase-3 protein was elevated.Conclusion: ATRA can inhibit the proliferation of lung cancer cell A549 and induce cell apoptosis by upregulating the expression of activated Caspase-3 protein.