| 重组水蛭素Ⅲ的工程菌的培养及高密度发酵 |
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| 引用本文： | 崔莉,谭树华,吴梧桐.重组水蛭素Ⅲ的工程菌的培养及高密度发酵[J].药物生物技术,2004,11(1):22-24. |
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| 作者姓名： | 崔莉 谭树华 吴梧桐 |
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| 作者单位： | 中国药科大学生命科学与技术学院,江苏,南京,210009 |
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| 基金项目： | 国家自然科学基金资助,No .3 0 0 0 0 2 14，国家教育部霍英东教育基金会青年教师基金项目No .810 3 2 |
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| 摘 要： | 为提高重组水蛭素Ⅲ工程菌的产水蛭素量，采用正交实验法确定了较佳培养基组分，优化了摇瓶发酵工艺和发酵条件，工程菌摇瓶水蛭素产量稳定在2000ATU／ml左右，30L发酵罐批式发酵水蛭素产量达到4000ATU／ml，补料一批式发酵水蛭素产量达到8000ATU／ml。
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| 关 键 词： | 水蛭素Ⅲ 大肠杆菌 发酵 |
| 文章编号： | 1005-8915(2004)01-0022-03 |
Cultivation and High-Density Fermentation of Recombinant Hirudin Ⅲ in Engineered E.coli |
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| CUI Li,TAN Shu-hua,WU Wu-tong.Cultivation and High-Density Fermentation of Recombinant Hirudin Ⅲ in Engineered E.coli[J].Pharmaceutical Biotechnology,2004,11(1):22-24. |
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| Authors: | CUI Li TAN Shu-hua WU Wu-tong |
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| Institution: | CUI Li,TAN Shu-hua,WU Wu-tong* |
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| Abstract: | In order to improve the hirudin production by an engineered Escherichia coli, a suitable culture medium was selected by orthogonal experimental design, some fermentation conditions were optimized. Hirudin was maintained 2 000 ATU/ml in flask and 4 000 ATU/ml in a 30 L fermentor. However, hirudin was further enhanced to 8 000 ATU/ml in a 30 L fed-batch fermentor. |
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