Suppressive effect of Petasites japonicus extract on ovalbumin-induced airway inflammation in an asthmatic mouse model |
| |
Authors: | Lee Ji-Sook Yang Eun Ju Yun Chi-Young Kim Dong-Hee Kim In Sik |
| |
Affiliation: | a Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan 570-750, Republic of Korea b Department of Biology, College of Natural Sciences, Daejeon University, Daejeon 300-716, Republic of Korea c Department of Biomedical Laboratory Science, School of Medicine, Eulji University, 143-5, Yeuongdu-dong, Jung-gu, Daejeon 301-832, Republic of Korea d Department of Pathology, College of Oriental Medicine, Daejeon University, Daejeon 300-716, Republic of Korea |
| |
Abstract: | Aim of the studyAsthma is a disease marked by airway inflammation. Petasites japonicus (Pj) is known as an herb for treating asthma, oxidant stress and gastric ulcer in traditional Oriental medicine. In this study, the inhibitory effects of Pj extract on asthmatic responses were examined both in vitro and in vivo.Materials and methodsThe Pj extract was acquired from whole plants of Petasites japonicus using 80% ethanol. Cytotoxicity of the Pj extract on Jurkat cells and THP-1 cells was determined using MTT assay. ELISA was performed to determine the expression levels of cytokines, chemokines, and IgE. BALB/c mice were used for an OVA-induced asthmatic mouse model. Reactive oxygen species (ROS) production was stained with 2′,7′-dichlorofluorescein diacetate and measured by fluorescence-activated cell sorting analysis. The effects of the Pj extract on leukocyte infiltration and mucus production were determined using periodic acid-Schiff staining as well as hematoxylin and eosin staining.ResultsThe Pj extract inhibits the increased release of interleukin (IL)-2, IL-4, IL-5, IL-13, and TNF-α due to house dust mite in Jurkat cells and blocks IL-6 expression in THP-1 cells without cytotoxicity. In the asthmatic mouse model, the Pj extract inhibits eosinophil infiltration, mucus hypersecretion, and IL-5 level in bronchoalveolar lavage (BAL) fluid, and it has a scavenging effect on ROS production of cells in BAL fluid.ConclusionThe Pj extract has suppressive properties for the pathogenesis of airway inflammation and may be used as a potent agent for the treatment of asthma. |
| |
Keywords: | Pj, Petasites japonicus OVA, ovalbumin BAL, bronchoalveolar lavage IgE, immunoglobulin E ELISA, enzyme-linked immunosorbent assay ROS, reactive oxygen species Dex, dexamethasone |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|