| 8-硝基白杨素诱导人结肠癌HT-29细胞凋亡 |
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| 引用本文： | 伍尤华,曹建国.8-硝基白杨素诱导人结肠癌HT-29细胞凋亡[J].中国现代医学杂志,2008,18(23):3443-3445. |
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| 作者姓名： | 伍尤华 曹建国 |
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| 作者单位： | 1. 南华大学附属第一医院肿瘤内科,湖南,衡阳,421001
2. 南华大学医学院肿瘤研究所,湖南,衡阳,421001 |
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| 基金项目： | 湖南省自然科学基金(No:03JJY5009); 湖南省卫生厅医药科研项目(No:B2006-141;C2005-036) |
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| 摘 要： | 目的 观察8-硝基白杨素(NOChR)诱导人结肠癌HT-29细胞凋亡作用.方法 MTT比色法测定细胞增殖活性;流式细胞分析术检测细胞凋亡率;琼脂糖凝胶电泳观察DNA梯形条带.结果 NOChR抑制人结肠癌(HT-29)细胞增殖,呈浓度依赖性,其IC50值为1.78μM;NOChR具有诱导HT-29细胞凋亡作用;PPAR γ特异性阻断剂(GW9662)能有效拮抗NOChR诱导HT-29细胞凋亡作用.结论 NOChR诱导HT-29细胞凋亡作用与其活化PPAR γ相关.
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| 关 键 词： | 结肠癌 白杨素 白杨素衍生物 凋亡 |
Induction of apoptosis of human colon cancer HT-29 cells by 5,7-Dihydroxy-8-nitrochrysin |
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| WU You-hua
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CAO Jian-Guo.Induction of apoptosis of human colon cancer HT-29 cells by 5,7-Dihydroxy-8-nitrochrysin[J].China Journal of Modern Medicine,2008,18(23):3443-3445. |
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| Authors: | WU You-hua CAO Jian-Guo |
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| Institution: | WU You-hua1,CAO Jian-Guo2(1.Department of Oncology,First Affiliated Hospital,Nahua University,Hengyang,Hunan 421001,P.R.China,2.Institute of Cancer Research,Nanhua University,P.R.China) |
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| Abstract: | ObjectiveTo investigate induction of apoptosis of human colon cancer HT-29 cells by 5,7-dihydrox-8-nitrochrysin in vitro. MethodsThe proliferative activity of HT-29 cells treated with 5,7-dihydrox-8-nitrochrysin was measured using 3-(4,5-dimethylthiazol-2-yl) -2,5-dipheny tertrazolium blue(MTT) colorimetric assay. HT-29 cell apoptotic percentage was detected by flow cytometry with PI staining. DNA ladder bands were observed by DNA agarose gel electrophoresis. ResultsMTT assay suggested that 5,7-dihydrox-8-n... |
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| Keywords: | human colon cancer chrysin chrysin derivatives apoptosis |
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