妊娠与非妊娠妇女生殖道肺炎支原体感染的研究

目的：了解我国妊娠与非妊娠妇女生殖道肺炎支原体（Mpn)感染状况。方法：应用套式聚合酶链式反应（nPCR)、选择性培养和全自动DNA序列测定技术对100例妊娠妇女和100例非妊娠妇女的阴道拭子标本进行检测。结果：Mpn nPCR检测妊娠妇女阴性率为6％，非妊娠妇女为3％。Mpn nPCR呈阳性的标本经选择性培养均分离到Mpn。1例Mpn nPCR阳性产物经全自动DNA序列测定与Mpn标准株序列完全一致。结论：我国妊娠与非妊娠妇女生殖道均存在Mpn感染。

Study of mycoplasma pneumoniae infection in pregnant and non - pregnant women

Objective:To study mycoplasma pneumoniae(Mpn) infection in the genital path of pregnant women and non-pregnant women in china.Methods Applying nested Polymerase Chain Reaction(nPCR)?selective cultrure and whole automatism DNA sequence mensuration technique determined vagina speciens from 100 pregnant women and 100 non-pregnant women.Results Postive rate of Mpn in pregnant women was 6% with nPCR;Postive rate of Mpn in non-pregnant women was 3% with nPCR.Among the postive cases,Mpn were detected with selective culture.Sequence of 1 Mpn nPCR postive product was entire consistent with MPn standard.Conclution There exsists Mpn infection in the genital path of pregnant women and non-pregnant women in china.