AGL提取物总黄酮的部分药效学研究

目的：探讨碱-酸法提取的AGL总黄酮的毒性及其对SD大鼠血压及兔动脉粥样硬化动物模型的影响。方法：（1）选用SD大鼠，静脉推注AGL总黄酮（1mg／kg，2mg／kg），观察血压波动变化。（2）选用新西兰兔，高脂饲料喂养形成动脉粥样硬化动物模型，灌胃给予AGI。总黄酮（5mg／kg），观察血清总胆固醇（TC）、低密度脂蛋白-胆固醇（LDL-C）、高密度脂蛋白～胆固醇（HDL-C）、甘油三酯（TG）、C反应蛋白（CRP）及主动脉的病理学变化。结果：（1）小鼠对AGI。总黄酮最大耐受剂量为6g／kg。（2）AGL总黄酮（1mg／kg，2mg／kg）对SD大鼠血压的影响差异无统计学意义（P〉0．05）。（3）AGL总黄酮（5mg／kg）对高脂饲料喂养形成的兔动脉粥样硬化动物模型的影响：AGL总黄酮组血清TC、LDL、HDL水平与模型组比较，差异无统计学意义（P〉0．05）；AGL总黄酮组血清TG、CRP水平虽有升高，但与模型组比较差异无统计学意义（P〉0．05）。病理学检查结果表明，主动脉内膜可见泡沫细胞、粉红色无定性物质。结论：用碱-酸法提取的AGL总黄酮对大鼠血压和动脉粥样硬化动物模型无显著影响。

Pharmacodynamic studies on the extraction of AGL flavonoids

Objective: To investigate the acute toxicity of AGL flavonoids(extracted by the basic-acid method) and its effect on blood pressure and arteriosclerosis animal models.Methods:(1) Changes in blood pressure were observed by intravenous injections of AGL flavonoids(1 mg/kg, 2 mg/kg) in rats.(2) Experimental arteriosclerosis animal model were established in New Zealand albino rabbits by hyperlipoidal food,then AGL flavonoids were(5 mg/kg) given orally;Blood samples were collected to assess serum TC,LDL, TG,HDL,CRP and dimension of aortic changes in the histopathology.Results:(1)AGL flavonoids' maximum tolerance dose was 6 g/kg.(2) AGL Flavonoids showed no significant changes on blood pressure(P>0.05).(3) Compared with the hyperlipoidal food group,AGL flavonoids had no significant change in serum TC,LDL,HDL,but serum TG,CRP is high(P>0.05).Foam cells and atherosclerotic plaques were observed in aortic intima in the AGL flavonoids group.Conclusions: It is concluded that AGL flavonoids extracted by the basic-acid method possess no marked effects on blood pressure and arteriosclerosis models in rabbit.