HPLC法同时测定舒筋通络酒中两种有效成分的含量

目的:建立高效液相色谱法同时测定舒筋通络酒中升麻苷和5-O-甲基维斯阿米醇苷含量的方法。方法:色谱柱为Lichrospher C18(150 mm×4.6 mm,10μm);流动相为乙腈-0.05%磷酸(12∶88);检测波长为254 nm;柱温为40℃;流速为1.0ml.min-1。结果:升麻苷在0.098～0.491μg范围内线性关系良好(r=0.999 9);5-O-甲基维斯阿米醇苷在0.135～0.675μg范围内线性关系良好(r=0.999 8),平均回收率分别为98.41%(RSD=1.84%),99.28%(RSD=1.56%)。结论:本法简单、方便、准确,可测定该制剂升麻苷、5-O-甲基维斯阿米醇苷的含量。

Simultaneous Determination of Two Effective Components in Shujin Tongluo Spirits by HPLC

Objective： To establish a method for the simultaneous determination of prim-O-glucosylcimifugin and 5-O-methylvisammioside in Shujin Tongluo spirits by HPLC.Method： The sample was separated on a Lichrospher C18（150 mm×4.6 mm,10 μm） column with acetonitrile-0.05% phosphoric acid（12∶ 88） as the mobile phase.The detection wavelength was 254 nm,the column temperature was 40℃ and the flow rate was 1.0 ml·min-1.Result： The linear range of prim-O-glucosylcimifugin was 0.098~0.491 μg（r=0.999 9） with the average recovery of 98.41% and RSD of 1.84%.The linear range of 5-O-methylvisammioside was 0.135~0.675 μg（r=0.999 8） with the average recovery of 99.28% and RSD of 1.56%.Conclusion： The method is simple,convenient and accurate,and can be used in the determination of prim-O-glucosylcimifugin and 5-O-methylvisammioside.